HISTOPATHOLOGY Flashcards
Enzymes used for antigen retrieval in IHC
1. Phosphatase (AP – FOR LABELLING
ANTIBODY)
2. Trypsin
3. Lipase
4. Protease
2 & 4 AND PEPSIN
Which of the following statements is incorrect?
NONE OF THESE
All of the following are methods of antigen retrieval except:
INCINERATOR HEATING
Advantages of enzymatic IHC except:
NONE OF
THESE
Sources of polyclonal antibodies
1. Sheep
2. Goat
3. Rabbit
4. Mice
MICE
Compatible counterstains for DAB
1. Brilliant green
2. Methyl violet
3. Eosin
4. Safranin
NONE OF THESE
If alkaline phosphate was used to label antibodies in IHC which of the following can be used?
1. DAB
2. Fast Red TR salt
3. Amino Ethyl Carbazole
4. BCIP
2 & 4
Which of the following chromogens that when used will produce a bluish violet or black violet color?
BCIP
In IHC, what is the incubation time and
temperature to link antibody with enzyme?
30-60 MINUTES AT RT
Which of the following is the most commonly used enzyme label in IHC?
HRP
In IHC, which form of hematoxylin that when used tissue morphology are well defined?
HARRIS
Acceptable specimens for IHC except:
NONE OF
THESE
Which of the following is an oncofetal antigen that is very useful differentiating mesothelioma and adenocarcinoma.
CEA
Which is highly specific for myogenic tumor?
DESMIN
Which of the following is the marker for
choriocarcinoma?
HCG
The tumor marker that will confirm diagnosis of
astrocytoma?
GFAP
An epithelial tumor marker which is also positive in certain pancreatic and salivary gland cancer
PSA
Conditions that will stan positive for vimentin.
ALL OF THESE
Which of the following is synthesized by placental syncitioltrophoblast?
HCG
All are intermediate filament markers except:
TTF-1
Which of the following is more common in
carcinomas of the colon and stomach?
CK 20
Which can be helpful in determining tumor site?
EMA
Most common form of antibody used in IHC?
IgG
True of IHC except:
D. SPECIMEN FOR IHC IS
LIMITED ONLY TO PRICESSED SPECIMEN LIKE
PARAFFIN EMBEDDED TISSUES
Uses of this chromogen can lead to production of brick red color.
AEC
Cells found in cervico vaginal smear with a
diameter of 15-30u
PARABASAL CELLS
Which cell has basophilic vacuolated cytoplasm.
INTERMEDIATE CELLS
In Pap’s staining of non-gynecological specimens. Which form of hematoxylin is used as nuclear stain?
HARRIS
Which of the following are used as counterstains in Pap’s staining?
2 & 4
Which of the following cells in cervico vaginal
smear is shed in response to ovarian hormone?
ENDOMETRIAL CELLS
These are boat shaped cells with a tendency to fold or curl on edges.
NAVICULAR CELLS
Which may assume a “honeycomb” appearance?
ENDOCERVICAL CELLS
The presence of these cells suggests a combine
estrogen-progesterone effect?
NAVICULAR CELLS
Predominance of which of the following cells is
noted following childbirth?
PARABASAL CELLS
These are polygonal squamous cells measuring 45-50um in diameter.
SUPERFICIAL CELLS
In Pap’s staining of gynecological specimens. Which form of hematoxylin is used as nuclear stain?
HARRIS
In Pap’s staining of the following will that when
used will stain the cytoplasm of other cells
including non-keratinized squamous cells.
LIGHT GREEN SF
In cytochemistry, which can be used to stain
glycogen?
PERIODIC ACID SCHIFF
Which is considered to be that staining method of choice in exfoliative cytology.
PAPANICOLAOU
All of the following statement are correct except:
E. NONE OF THESE
