Histology Lecture 1 - Intro to Methods and Stains

Question 1

What charge does a basic dye carry?

Answer 1

A POSITIVE charge, because it has picked up a proton.

Question 2

What charge does an acidic dye carry?

Answer 2

A NEGATIVE charge, because it has lost a proton.

Question 3

Name two examples of basic dyes.

Answer 3

Toluidine blue and hematoxylin.

Question 4

Name an example of an acidic dye.

Answer 4

Eosin.

Question 5

What type of molecules have affinity for basic dyes?

Answer 5

Negatively charged molecules.

Question 6

What type of molecules have affinity for acidic dyes?

Answer 6

Positively charged molecules.

Question 7

What does a Periodic-acid Schiff stain dye?

Answer 7

Structures rich in carbohydrates.

Question 8

What do silver stains dye?

Answer 8

Nerve cell processes and reticular fibers

Question 9

What does Masson’s trichrome stain do?

Answer 9

It is a three-colored stain that distinguishes cells from their surrounding extracellular matrix.

Question 10

What is metachromasia?

Answer 10

A phenomenon that results in a change in dye color due to interaction between basic dye (like toluidine blue) and polyanions.