Histology Exam 1 Flashcards
what are the functions of cartilage?
provide flexible support
articular surfaces of joints
temporary template for skeleton
spaces chondrocytes sit in
lacunae
what are the ECM components of cartilage?
type 2 collagen
hyaluronic acid
GAGs
dense outer layer of cartilage- what are its two layers
perichondrium- outer fibrous layer and inner cell layer with progenitor cells
what is interstitial growth?
growth from within- chondrocytes make matrix around themselves and then get trapped
group of chondrocytes in a lacunae
isogenous group
what is appositional growth?
growth on a surface- progenitor cells in perichondrium differentiate into chondroblasts and make new matrix
how does cartilage develop?
interstitial growth to begin, then appositional is used for repair
where is hyaline cartilage?
upper airway (trachea, bronchii, larynx)
joints
ventral ribs
early skeleton
what is hyaline cartilage made of?
type 2 collagen
where is elastic cartilage?
epiglottis
larynx
ear
eustachian tube
what is elastic cartilage made of
type 2 collagen
elastin fibers
where is fibrocartilage
IVD
pubic symphysis
what is fibrocartilage made of
type 1 and 2 collagen
where does cartilage get nutrients from?
surrounding connective tissue
bone matrix
collage type 1
GAGs
proteoglycans
non collagenous proteins
where do osteoblasts come from?
osteoprogenitor cells
where do osteoclasts come from?
blood cell precursor, monocyte lineage
where do osteocytes come from?
osteoblasts that get stuck in the matrix
what type of marrow is active?
red
what type of marrow is inactive?
yellow
what layer of bone has progenitor cells in it? where is it?
endosteum- between compact and spongy bone
what is the layer on the outside of bone?
periosteum
where are osteoblasts found? what do they look like?
cells in a line on the surface- do appositional growth
what are the phases of osteocytes?
quiescent- resting
formative- signal bone deposition
resorptive- signal bone resorption
which type of bone cells is multinucleated?
osteoclasts
what are hawship canals?
holes osteoclasts sit in
how are osteoclasts made?
osteoblasts produce MCSF which recruits monocyte stem cells to bone and RANKL
osteoclast precursor cells have RANK, RANKL binds to RANK and induces differntiation
what is osteoprotegrin?
blocks RANK and inhibits osteoclast maturation
steps of bone resorption
- osteoclasts target bone via chemotaxis
- attach via cell adhering molecules
- seal to bone forming a resorptive cavity
- release H to dissolve minerals (acidic environment)
- lysosomes degrade collagen, MMPs degrade other things
what are lamellae?
concentric layers of bone surrounding Haversian cannals
steps of bone formation
osteiod is deposited (unmineralized bone)
osteoblasts release stuff that attracts Ca and PO4-
hydroxyapetite crystals form
continuous remodeling
what does osteoid look like
very irregularly arranged collagen
what happens to the osteoid?
reabsorbed by osteoclasts
what are the 2 types of bone formation?
intramembranous
endochondral
describe intramembranous bone formation
osteoblasts secrete matrix which is then mineralized
the bone is resorbed and reshaped
what is intramembranous bone formation used for?
flat bones
describe endochondral bone formation
cartilage forms first and then is replaced by bone
what is endochondral bone formation used for
long bones
steps in endochondral bone formation
- temporary hyaline cartilage skeleton formed which is surrounded by perichondrium
- bone formation starts in specific areas and moves outwards
where is the primary ossification center?
in the middle of the diaphysis
where is the secondary ossification center
epiphyses
what does parathyroid hormone related peptide do?
stimulates chondrocyte proliferation in the temporary cartilaginous skeleton
what causes dwarfism?
defects in Indian hedgehog protein- chondrocytes don’t proliferate enough so bones aren’t long eough
what does indian hedgehog protein do?
increases PHrP receptors on chondrocytes to delay hypertrophy until the bone gets to be the correct length
what does fibroblast growth factor do?
inhibits cartilage growth
what causes achondroplastic dwarfism?
gain of function mutation in FGF- increased FGF binding inhibits cartilage growth, so bones aren’t correct length
what regulates periosteal collar formation?
indian hedgehog protein
what is rickets?
vitamin D defiency in children resulting in soft bones due to defects in mineralization
what is osteomalacia?
vitamin D deficicy in adults resulting in demineralization of bone–>soft bones
what is osteoporosis?
increased bone fragility due to estrogen deficiency–> increased bone resorption without increased formation
what is Paget’s disease
osteoblasts overcompensate for high osteoclast activity by laying down a lot of woven bone resulting in abnormally large bones that are prone to fracture because the osteoid isn’t mineralized
synarthrosis?
immovable joint
amphiarthrosis
slightly movable joint
diarthrosis
movement
osteoarthritis
excessive wear of articular cartilage with secondary inflammation
rheumatoid arthritis
chronic systemic inflammation leading to thickening of synovial membrane
what is a pannus?
folds in synovial membrane from rheumatoid arthritis