HISTO stains Flashcards
If a tissue is acidophilic, what charge is the dye and what does it bind to and what color is it?
Na+, binds to cationic groups NH3+ amino groups, Red
What color will the cytoplasm and connective tissue fibers stain
red, acidophilic
If a tissue is basophilic, what charge is the dye and what does it bind to and what color is it?
Cl-, binds to anionic groups (HPO4), blue
What type of dye is typically used for acids?
eosin
What type of dye is typically used for bases?
hematoxylin
When using Hematoxylin and Eosin,What color will the Nucleus and rER stain?
blue
When using Hematoxylin and Eosin,What color will the cytoplasm stain?
varying shades of red
When using Hematoxylin and Eosin,What color will collagen stain?
very pale pink
When using Hematoxylin and Eosin,what color will cartilage and calcium stain?
dark blue
When using Hematoxylin and Eosin,What color will red blood cells stain?
bright red
When using Hematoxylin and Eosin,is the most common stains used in histology?
eosin and hematoxylin
What are trichrome stains good for?
collagen fibers and muscle
When using trichrome stains, what color will collagen appear? What about muscle?
light blue
Red
What do you use Mallory’s stain for?
differentiate pituitary cell types, but sometimes used for collagen
When using mallory’s stain, what color will collagen appear? What about elastin fibers?
blue
Pink,yellow or unstained
