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Question 1

What are the lenses for the optical component ?

Answer 1

Condenser
Objective
Eyepiece

Question 2

what is the resolving power?

Answer 2

the smallest distance between two structures at which they can be seen as separate objects

Question 3

What is the function of condenser ?

Answer 3

r focusing light on the object
to be studied

Question 4

What is the function of objective lens?

Answer 4

enlarging and projecting the
image of the object toward the observer

Question 5

what is the function of eyepiece ?

Answer 5

Increasing the image magnification and projecting
it onto the viewer’s eye

Question 6

what is the The critical factor in obtaining a crisp, detailed image with a light microscope ?

Answer 6

the resolving power

Question 7

what is The maximal resolving power of the
light microscope ?

Answer 7

0.2 μm مايكرو ميتر

Question 8

How much can you enlarge the images?

Answer 8

1000-1500 times

Question 9

WHAT VIRTUAL MICROSCOPY USED FOR?

Answer 9

STUDY OF BRIGHT FIELD MICROSCOPIC preparations

Question 10

how we can use the fluorescent compounds wITH affinity for specific cell macromolecules ?

Answer 10

as fluorescent stains

Question 11

what is the fluorescence ?

Answer 11

irradiate cellular substances with light of a proper wavelength they emit light with a longer wavelength

Question 12

in fluorescence
microscopy, tissue sections are usually irradiated with ?

Answer 12

(UV) light and visible portion of
the spectrum.

Question 13

how can we study the Unstained cells and tissue sections?

Answer 13

by using the modified
light microscopes LIKE Phase-Contrast

Question 14

why . Cellular detail is normally difficult to see
in unstained tissues ?

Answer 14

because all parts of the specimen have
roughly similar optical densities

Question 15

what Phase-contrast microscopy based on ?

Answer 15

light changes its speed when passing through cellular and extracellular structures with different refractive indices

Question 16

Why is light at different speeds in phase contrast micro?

Answer 16

to appear lighter or darker in relation to each other.in the structures

Question 17

what differential interference contrast microscopy with Nomarski optics produce

Answer 17

an image of living cells with a more apparent three-dimensional (3D

Question 18

Why do we use Confocal Microscopy ?

Answer 18

to Achieves high resolution and sharp focus

Question 19

why we can get high resolution and sharp focus with using confocal microscopy

Answer 19

Because the final incident light is a small point of high intensity light, often from a lase

Because there is a pinhole plate in front of the image detector.

Question 20

What is the function of polarizing microscopy

Answer 20

allows the recognition of stained or
unstained structures made of highly organized subunits.

Question 21

tissue structures containing oriented macromolecules
are located between the two polarizing filters, their repetitive structure rotates the axis of the light emerging from the
polarizer and they appear as bright structures against a dark
background

Answer 21

فهم

Question 22

what is the birefringence ?

Answer 22

The ability to rotate the direction
of vibration of polarized light

Question 23

example of birefringence ?

Answer 23

cellulose collagen micro-tubules actin filaments

Question 24

EXAMPLE FOR FLUORESCENT STAINS ?

Answer 24

ACRIDINE ORANGE(DNA /RNA) AND DAPI stain specifically bind DNA and
are used to stain cell nuclei

Question 25

electron microscopes are based on ?

Answer 25

the interaction of tissue components with beams of electrons

Question 26

WHAT IS THE TYPE OF electron microscope?

Answer 26

Transmission
Scanning