High Yield Specific Examples Flashcards
Specific examples of concepts from lecture (aka "this is one of my favorite enzymes")
What keeps the movement of molecules in/out of RBCs neutral in isohydric transport?
Chloride ions
What is the structure, function, role of Glutathione?
Antioxidanct, non-alpha Carbon peptide bond, sulfhydryl group is oxidized/reduced
How would you screen for metabolites in fetal blood?
LC-MS
How would you identify Hepatitis-B marker in serum?
ELISA
How would you identify protein expression in tissue sample?
Gel Electrophoresis
What is a major secondary structure for NAD+ Dependent Dehydrogenases?
Rossman Fold
What forms the B-turns in Collagen Cross Linking?
Proline-Cis-Trans Isomerases
What is the role of HSPs in protein folding?
Arrange secondary structure
What is the role of Chaperones in protein folding?
Move hydrophobic residues to interior, hydrophilic residues to exterior
What enzyme stabilizes tert/quat structure via cystine bridge?
Disulfide Isomerase
What two PTMs are key for collagen cross links?
HydroxyPro / HydroxyLys
What enzyme does hydroxyPro require, and what disease is it related to?
Proxyl Hydroxylase - enzyme requires Vitamin A, and a deficiency can result in Scurvy
What enzyme does hydroxyLys require, and what disease is it related to?
Lysyl Oxidase - lathyrism is inhibition of enzyme
What enzyme regulates Elastin breakdown, and what disease(s) is it related to?
alpha-1 Antitrypsin inhibits elastase, smoking can deactivate it and elastase can degrade tissues (respiratory issues)
What are cycophilins and what do they do?
They are an inhibitor of proline-cis-trans isomerases, and they are used as immuno suppressants
