Hematology Week 2: Lab Testing of Hematologic Neoplasms Flashcards

1
Q

Lab Tests of Hematologic Neoplasms

7 listed

A
  • Morphology
  • Cytochemistry
  • Flow Cytometry
  • Immunohistochemistry
  • Chromosomes
  • FISH
  • Molecular
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2
Q

Which is a blast?

A

C

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3
Q

Question 1

A

A. Can detect multiple antigens on a single cell

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4
Q

How does Flow Cytometry work?

A

intensity of signal can denote quantity

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5
Q

Analyzing Flow Cytometry Results

A
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6
Q

CD45 Expressed by?

A

all Hematopoietic cells

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7
Q

CD19 Expressed by?

A

B cells

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8
Q

CD20 Expressed by?

A

B cells

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9
Q

Kappa Expressed by?

A

B cells

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10
Q

Lambda Expressed by?

A

B cells

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11
Q

CD3 Expressed by?

A

T cells

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12
Q

CD4 Expressed by?

A

T cells

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13
Q

CD5 Expressed by?

A

T cells

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14
Q

CD8 Expressed by?

A

T cells

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15
Q

MPO expressed by?

A

Myeloid Cells

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16
Q

MPO AKA

A

Myeloperoxidase

17
Q

CD33 Expressed by?

A

Myeloid Cells

18
Q

CD34 Expressed by?

A

Immature cells (blasts)

19
Q

TdT Expressed by?

A

Immature cells (Blasts)

20
Q

Question 2

A

C

21
Q

TdT AKA

A

Terminal Deoxytransferase

22
Q

Auer Rod indicates?

A
  • Only arises in myeloid neoplasm
  • in abnormal cells fusion of abnormal granules form Auer Rod
23
Q

Immunohistochemistry

A
24
Q

Question 3

A

A CD19

25
Q

Question 4

A

A - B-cell Lymphoma

26
Q

clonality analysis

A

mixture would be multiple peaks clonal would be strong single peak

27
Q

Question 5

A

B

Acute Lymphoblastic Leukemia

28
Q

Karyotype important

A

diagnostically but also prognostically

29
Q

Dual Fusion Fish

A