HDL AND TAG DETERMINATION Flashcards
LABORATORY EXPERIMENT
Components used in Separation Method:
500 uL of sample (SERUM)
50 uL of HDL precipitating agent
In separation method of HDL determination, disturbing the sediment while aspirating will lead to
Falsely decrease
Reference method for Lipoprotein
Ultracentrifugation
HDL Standard
50 mg/mL
Wavelength
500 nm
Expected values of HDL for males:
30-70 mg/mL
Expected values of HDL for females:
30-85 mg/mL
Measurement of TAG levels, when performed in conjunction with other lipid assays, proves useful in the diagnosis of
Primary and Secondary Hyperlipoproteinemia
Enzyme in Secondary reaction (TAG)
Glycerol Kinase
Enzyme in Tertiary reaction (TAG)
GlycerylPhosphate Oxidase (GPO)
Enzyme in Quartery reaction (TAG)
Peroxidase
End product in Secondary reaction (TAG)
Glycerol-3-Phosphate (G-3-P) and Adenosine-5’-Diphosphate (ADP)
End product in Tertiary reaction (TAG)
Dihydroxyacetone Phosphate (DAP) and Hydrogen Peroxide (H2O2)
End product in Quartery reaction (TAG)
Quinoneimine Complex (Red)
Components of Triglyceride Standard:
Glycerol with surfactant and 0.1% Sodium azideas preservative
Expected values for Triglycerides in Conventional unit
44-148 mg/dL
Expected values for Triglycerides in SI unit
0.50-1.67 mmol/L
Equation to be used when TAG level is <400 mg/dL
Friedwald Equation
In Friedwald Equation, if TAG is in Conventional unit, formula for VLDL to be used is
VLDL= TAG/5 (mg/dL)
In Friedwald Equation, if TAG is in SI unit, formula for VLDL to be used is
VLDL= TAG/ 2.175 (mmol/L)
Formula for LDL
LDL= Total Cholesterol - (HDL + VLDL)
Equation to be used when TAG level is >400 mg/dL
Delong Equation
In Delong Equation, if TAG is in Conventional unit, formula for VLDL to be used is
VLDL= TAG/ 6.5 (mg/dL)
In Delong Equation, if TAG is in SI unit, formula for VLDL to be used is
VLDL= TAG/ 2.825 (mmol/L)
