GLUCOSE DETERMINATION Flashcards
Laboratory Experiment
1
Q
Enzyme in Primary Reaction
A
Glucose oxidase (GOD)
2
Q
Enzyme in Secondary Reaction
A
Peroxidase (POD)
3
Q
End product in the Primary Reaction
A
Gluconic acid and Hydrogen Peroxide (H2O2)
4
Q
End product in Secondary Reaction
A
Quinone Complex (Red-Violet)
5
Q
Reagents used in Glucose Determination
A
Phospate Buffer
Phenol
4-aminoantipyrine
Glucose oxidase
Peroxidase
6
Q
Glucose Standard (mg/ml)
A
100
7
Q
Wavelength
A
500nm
8
Q
Reference range of Serum/Plasma in SI unit:
A
3.89-5.83 mmol/L
9
Q
Reference range of CSF in SI unit
A
2.22-4.17 mmol/L
10
Q
Reference range of Serum/Plasma in Conventional unit:
A
70-105 mg/dL
11
Q
Reference range of CSF in Conventional unit:
A
40-75 mg/dL
