ANALYTICAL METHODS AND INSTRUMENTATION

Question 1

LIGHT WAVES THEORY:
> The light was made up of tiny particles
> The light moves in a straight direction in a vacuum.

Answer 1

ISAAC NEWTON

Question 2

LIGHT WAVES THEORY:
> Described as photons of energy travelling in waves
> Planck’s formula: E=hv; E= is the energy of a photon in Joules, h= constant (6.626 x 10^34 erg sec)

Answer 2

ELECTROMAGNETIC RADIATION

Question 3

LIGHT WAVES THEORY:
> Proposed a wave theory of light. Particles of light move in a wave-like manner

Answer 3

CHRISTIAN HUYGENS

Question 4

TRANSMITTED VIA ELECTROMAGNETIC WAVES THAT ARE CHARACTERIZED BY THEIR FREQUENCY AND WAVELENGTH

Answer 4

ENERGY

Question 5

THE DISTANCE BETWEEN TWO SUCCESSIVE PEAKS

Answer 5

WAVELENGTH

Question 6

DISCRETE PACKETS OF ENERGY OR PARTICLES

Answer 6

PHOTONS

Question 7

THE NUMBER OF VIBRATIONS OF WAVE MOTION PER SECOND

Answer 7

FREQUENCY

Question 8

WHEN WAVES CHANGES DIRECTION BECAUSE IT BOUNCES OFF A SURFACE

Answer 8

REFLECTION

Question 9

BENDING OF A RAY WHEN IT PASSES AT AN ANGLE FROM ONE MEDIUM INTO ANOTHER IN WHICH ITS SPEED IS DIFFERENT

Answer 9

REFRACTION

Question 10

LIGHT ENCOUNTERS A SMALL PARTICLE THE ELECTROMAGENTIC FIELDS CREATED BY THE MOTION OF THE PARTICLE’S ELECTRON MAKES THE LIGHT CHANGES DIRECTION

Answer 10

SCATTERING

Question 11

DECREASING WAVELENGTH

Answer 11

INCREASING FREQUENCY

Question 12

THE LOWER THE WAVE FREQUENCY

Answer 12

THE LONGER THE WAVELENGTH

Question 13

STATES THAT THE CONCENTRATION OF A SUBSTANCE IS DIRECTLY PROPORTIONAL TO THE AMOUNT OF LIGHT ABSORBED OR INVERSELY PROPORTIONAL TO THE LOGARITHM OF THE TRANSMITTED LIGHT

Answer 13

BEER’S LAW

Question 14

AMOUNT OF LIGHT ABSORBED.

Answer 14

ABSORBANCE

Question 15

AMOUNT OF LIGHT TRANSMITTED

Answer 15

TRANSMITTANCE

Question 16

Involves measurement of the light transmitted by a solution to determine the concentration of the light-absorbing substances in the solution

Answer 16

SPECTROPHOTOMETRY

Question 17

The device used to measure the wavelengths of the light or the intensity of radiation

Answer 17

SPECTROMETER

Question 18

Is an analytical instrument that is used to quantitatively measure the transmission or reflection of visible light, UV light, or infrared light.

Answer 18

SPECTROPHOTOMETER

Question 19

SPECTROPHOTOMETRY:
splits the monochromatic light into two components: one beam passes through sample, and other through a reference solution or blank

Answer 19

DOUBLE BEAM SPECTROPHOTOMETRY

Question 20

TYPES OF DOUBLE BEAM SPECTROPHOTOMETRY:
with 2 photodetectors, for the sample beam and reference beam

Answer 20

DOUBLE-BEAM IN SPACE

Question 21

TYPES OF DOUBLE BEAM SPECTROPHOTOMETRY:
1 photodetector; passes the monochromatic light through the sample cuvette then through the reference cuvette

Answer 21

DOUBLE-BEAM IN TIME

Question 22

SPECTROPHOTOMETRY:
Simplest type of an absorption spectrophotometer.
Designed to make one measurement at a time at one specified wavelength

Answer 22

SINGLE BEAM SPECTROPHOTOMETER

Question 23

SPECTROPHOTOMETRY:
It provides polychromatic light and must generate sufficient radiant energy or power to measure the analyte of interest

Answer 23

LIGHT/RADIANT SOURCE

Question 24

TYPES OF LIGHT SOURCE:
emits radiation that changes in intensity; widely used in the laboratory.

Answer 24

CONTINUUM SOURCE

Question 25

TYPES OF LIGHT SOURCE:
emits limited radiation and wavelength.

Answer 25

LINE SOURCE