guest lecture - TRIM in duck

Question 1

influenza + its natural host & reservoir, the duck

Answer 1

ducks and waterfowl are natural reservoir of avian influenza virus

• less severe symptoms
• innate immunity provides protection

Question 2

what are trim proteins?

Answer 2

tripartite motif proteins.

• modulate immune function or cell cycle regulation
• antiviral: innate immune signalling activation or physical restriction of viral particles

Question 3

Monkey TRIM5alpha - HIV?

Answer 3

binds HIV prevents infection

Question 4

3 project goals

Answer 4

1. determine TRIM proteins involved in influence infection in ducks using global transcriptomic analysis
2. determine mechanism of TRIM2-L in innate pathway modulation and viral restriction
3. determine if duck TRIM32 directly restricts influenza

Question 5

1. determine TRIM proteins involved in influence infection in ducks using global transcriptomic analysis

Answer 5

studying RNa transcripts produced by the genome

- comparing TRIM transcript levels in infected + uninfected duck tissue

Question 6

determine mechanism of TRIM2-L in innate pathway modulation and viral restriction

Answer 6

TRIM27L duck specific. Not in chicken

upregulated during infleunza infection in immune specific organs

Question 7

TRIM27-L restricts influenza A how?

Answer 7

only when activated innate immune system.

doesnt work if alone.

Question 8

determine if duck TRIM32 directly restricts influenza

Answer 8

huTRIM32 binds + degrades influenza protein PB1

• not strain specific
• does duTRIM32 have same function?

Question 9

duck TRIM32 function

Answer 9

co-localizes with influence protein PB1 in nucleus

- restricts influenza virus in chickens