Growth requirement And nutrition Flashcards
Example of macronutrients
Proteins
Carbohydrates
Required in small amounts
Involved in enzyme function & maintenance of protein structures
Micronutrients
Examples of micronutrients
Manganese
Zinc
Nickel
Basic elements
CHON
Certain strains of bacteria need small amounts of
Phosphorus
Sulfur
Microbes can acquire nutrients in various ways
Based on carbon and energy source
Autotrophs
Photo Autotrophs
Chemo autotrophs
Carbon source - co2
Energy source - non living environment
Autotrophs
Have chlorophyll
Use light and co2
Oxygen is byproduct
Photo autotrophs
Uses hydrogen & sulfur
Chemo autotrophs
Carbon source - organic
Energy source - other organism/ sunlight
Heterotrophs
Use organic compounds
Photoheterotrophs
Most common
Aerobic and anaerobia
Chemoheterotrophs
Influences of microbial growth
Cp-NOTE
grows to the surface, where the oxygen is
Obligate aerobes
Example of obligate aerobes
Pseudomonas
Require small amount of oxygen
20% O2
Microaerophile
Example of microaerophile
Treponemapallidum
Grow well in the presence or absence of oxygen
Facultative aerobe
Example pf facultative aerobe
Enterobacteriaceae
Bottom dwellers
Not grow in the presence of oxygen
Obligate anaerobes
Source of energy of obligate anaerobes
Fermentation
Anaerobic respiration
Examples of obligate anaerobe
Clostridium
Bacterioides
Prevotella
Prophyromon
Grow in the presence of air but do not use it for respiration or energy
Aerotolerant
Example of aerotolerant
Strep pyogenes
Toxic forms of oxygen
Monoxide
Superoxide
Peroxide
Hydroxyl/radical
Temperature that may denature proteins
Cells may become fluid
Too high
Temperature that makes membrane and cells rigid and fragile
Too low
Heat loving organisms
Thermophiles
Moderate temperature
Mesophiles
Growth range of 10-50c or 20-40c
Our normal flora and pathogens
Mesophiles
Organisms that can endure heat
Thermoduric
Only these 2 produce spores
Clostridia
Bacillus
Growth range of 0-15c
Psychrophiles
Ideal pH for microbes
7-7.2
pH 1-5
Acidophiles
pH 5.5-8
Neutrophiles
pH 8.5-11.5
Alkalophiles
Live under extreme pressure
Barophiles
Continuous changing of medium
Chemostat
Autocount
Serial dilution method
Most powerful number count
MPN
Know how many bacteria you have
Cell counters
Measure Metabolic activity, dry weight
Spectrophotometry
Measure amount of DNA of present in a bacteria
Genetic methods
Required in large amounts
Role in cell structure and metabolism
Macronutrients
Example of photoautotrophs
Cyanobacteria
Example of chemoautotroph
Methanogens
Metabolizes the organic matter in dead organisms
Saprobe
Example of saprobe
Fungi
Bacteria
Utilizes tissue fluids of alive host
Parasite