Bacterial Metabolism And Genetics Flashcards
Describe anabolism
Biosynthesis
Building complex molecules
Requires ATP
Describe catabolism
Degradation
Breaking down complex molecules
Releases ATP
Example of precursor molecules
Glyceraldehyde 3 phosphate
Pyruvis acid
Acetyl group
Precursor for amino acids, carbohydrates ang triglycerides
G-3-P
Precursor for amino acid
Pyruvic acid
Proteins which are good examples of caralysts
Enzymes
What are catalysts
Chemicals that speed up the rate of chemical reaction
Check list of enzyme characteristics
Proteins Organic catalysts Lower the activation of energy Unique characteristics Provide reactive site Larger in size than substrates Do not become integrated into rxn process Not used up in the rxn Function in extermely low conc Limited by pH and temp Regulated by feedback and genetic mechanism
Consist of protein alone
Simple enzymes
Protein + non protein
Complex enzyme
Protein portion
Apoenzyme
Non protein portion
Co factor
Types of co factors
Metallic
Coenymes
Metallic co factors
M-I-C
Magnesium
Iron
Copper
Examples of coenzymes
Organic molecules
Vitamins
Part that accepts the substrate
Active site
Catalytic site
Breaks down hydrogen peroxide
Catalase + iron
Adds electrons to oxygen
Oxidase + iron copper
Transfers phosphate to glucose
Hexokinase + magnesium
Splits urea to ammonium
Urease + nikel
Reduces nitrate to nitrite
Nitrate reductase + molybdenum
Synthesis of dna
Dna polymerase complex + zinc magnesium
Enzymes transported extracellularly to become active
Exoenzymes
Retained intracellularly
Endoenzymes
Always present in constant amount regardless of the number of substrates
Constitutive enzymes
Not always present, only produced when substrate is present
Inducible enzymes
Synthesis or condensation reaction
Anabolic
Form covalent bonds
Require atp
Release 1mol of water
Hydrolysis reaction
Catabolic
Breakdown substrates
Require input of water
Transfer reactions by enzymes
Oxidation reduction Amino transferases Phosphotransferases Methyl transferases Decarboxylases
Chemically unstable enzymes
Labile
Weak bonds that maintain the shape of the enzymes are broken
Denaturation
Patterns of metabolic pathway
Linear
Cyclic
Branches
Differentiate competitive/noncompetitive/enzyme repression/enzyme induction
Competitive - same active site
Non competitive - may allosteric binding site
Repression - genetic level, control synthesis of key enzymes
Induction - enzymes are made only when substrates are present
Strep pyogenes
Streptokinase
Protease
Sk- digest blood clots, assist in invasion of wounds
Pase- flesh eating dx
Staph aureus
Lipase
Skin boils
Promote invasion of oil producing glands of the skin
P. Aeruginosa
Elastase
Collagenase
Respiratory & skin pathogen
Digest elastin
Digest collagen
Clostridium perfringens Lecithin C (lipase)
Gas gangrene, food poisoning
Damage cell membrane, cell death
Consume energy
Endergonic
Release energy
Exergonic
Bonds remained unchanged
Potential energy