Granulocytopoiesis Flashcards
Write out the process
Pluripotential stem cell
Myeloid committed stem cell (GEMM)
HPPCFC type 1
Hppcfc type 2
CFC-EOS| CFC-BAS| CFC-GM —> CFC G
Myeloblast
Promyelocyte
Myelocyte
Metamylocyte band
Segmented granulocyte
The cytoplasm of the myeloblast is usually empty of granules—although a few myeloblasts may show presence of few big granules called?
primary granules
At _______ stage, the cells become differentiable as a result of the presence of _____
Myelocyte
Secondary granules
The morphological characteristic of metamyelocyte is that the round nucleus of the myelocyte has become _____
Indented
not divide. It only further matures by further deepening of its nuclear indentation. This cell with nuclear indentation usually more than 1/3 of the cytoplasm is usually referred to as
band granulocyte.
The difference in the secondary granules relates essentially to the ______ dye that stain these granules.
Romanowski
The Romanowski dyes contain 2 types of dye in them
the basic dye that binds to acidic component of cells
an acidic dye that binds to the basic component of cells.
Examples of Romanowski dyes include
H&E stain
Leischmann stain
Wright stain
Jenner stain
Giemsa stain
Field stain.
The eosinophil granules are rich in basic granular content and are usually complexed with acidic component of Romanowski dye. The acidic components are usually red. Thus, the secondary granules of eosinophils are usually ______ when visualized under light microscopy.
Reddish
The granules of the basophils are rich in acidic proteins and are usually complexed with basophilic component of Romanoski dyes, and so they appear _____ under light microscopy
Bluish
The neutrophil granules contain ____________ macromolecules.
So, these granules complex with ________ dyes of the Romanowski stains. So, the granules do not appear reddish or bluish, but they choose a mixture of these colours and appear pinkish under light microscopy.
both acidic and basic
both basophilic and acidophilic
Or, in cases where the neutrophils are present in normal quantity but there are inherited granular abnormality such as in ______, phagocytosis will fail and the animal will face the risk of recurrent infections.
Wiskott-Aldrich syndrome
Functions of basophil
Alarm cells of the body
Mediate inflammation through production of histamine & other things
Function of eosinophils
Help to fight against parasitic infection as the CHARCOT LEYDEN GRANULES
Mediate killing of tissue parasites; dissolve their outer covering, immobilize & kill them
Function of neutrophils
mediate microbial phagocytosis. The neutrophils, like the monocytes, are also rich in cytoplasmic membrane Fc receptors as well as C3b receptors. As such, any microbial agent that has been opsonised (coated by an immunoglobulin or C3b) can be recognised by neutrophils, and then the neutrophils engulf such microbial agent. Thus, deficiency of neutrophils (neutropenia) is a condition that predisposes to severe bacteraemia and sepsis.
