gram staining

Question 1

what colour will gram positive bacteria stain

Answer 1

purple

Question 2

what colour will gram negative bacteria stain

Answer 2

pink

Question 3

true or false?
Hans gram was a German doctor who devised the gram stain procedure

Answer 3

false

Question 4

what is the first step of gram staining procedure

Answer 4

take sample of bacteria and smear on a glass slide

Question 5

second step

Answer 5

heat fix smeared bacteria to the slide

Question 6

third step

Answer 6

food slide with crystal voilet stain

Question 7

fourth step

Answer 7

wash slide with water to remove any extra crystal violet

Question 8

fifth step

Answer 8

flood slide with iodine stain

Question 9

sixth step

Answer 9

wash side with alcohol for decolorisation

Question 10

seventh step

Answer 10

flood slide with safranin for counterstaining

Question 11

eighth step

Answer 11

wash slide with water to remove excess safranin

Question 12

ninth step

Answer 12

blot dry and apply immersion oil for microscopic analysis

Question 13

what does gram negative bacteria typically do

Answer 13

mucus infections

Question 14

what does gram positive bacteria typically do

Answer 14

wound infections using very powerful toxins

Question 15

why is gram negitive bacteria less susceptible to some antibiotics eg penicillin

Answer 15

because the peptidoglycan layer is protected by lipopolysaccharide outer membrane which isn’t susceptible to some antibiotics

Question 16

what does a ram negative bacteria contain

Answer 16

thin cell wall
outer membrane
plasma membrane

Question 17

what does a gram positive bacteria contain

Answer 17

thick cell wall
plasma membrane