Gram positive II Flashcards

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1
Q

Important Corynebacterium species

A

Corynebacterium bovis, Corynebacterium renale, Corynebacterium pseudotuberculosis

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2
Q

Corynebacterium bovis causes

A

Subclinical mastitis in cattle

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3
Q

Usual habitat of Corynebacterium bovis

A

Teat cistern in cattle

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4
Q

Corynebacterium pseudotuberculosis hosts?

A

Sheep, goats, horses, cattle

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5
Q

Corynebacterium pseudotuberculosis biotypes?

A

Non-nitrate reducing biotype

Nitrate reducing biotype

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6
Q

Non-nitrate reducing biotype of Corynebacterium pseudotuberculosis causes?

A

Caseous lymphadenitis in sheep and goats

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7
Q

Usual habitat of non-nitrate reducing biotype of Corynebacterium pseudotuberculosis?

A

Skin and mucous membranes of small ruminants, environment

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8
Q

Nitrate reducing biotype of Corynebacterium pseudotuberculosis causes?

A

Ulcerative lymphangitis and abscesses in horses and cattle

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9
Q

Usual habitat of nitrate reducing biotype of Corynebacterium pseudotuberculosis?

A

Environment

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10
Q

Corynebacterium renale causes?

A

Cystitis and pyelonephritis in cattle

Ulcerative balanoposthitis in sheep and goats

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11
Q

Gram appearance of Corynebacterium species?

A

positive pleomorphic

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12
Q

General media requirements of Corynebacterium species?

A

Fastidious bacteria, require enrichened media

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13
Q

Majority of Corynebacterium species live where?

A

As commensals on mucous membranes

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14
Q

What type of infections Corynebacterium species cause?

A

Pyogenic infections

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15
Q

Motility of corynebacterium species?

A

Non motile

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16
Q

Atmosphere requirements of Corynebacterium species?

A

Facultative anaerobes

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17
Q

Corynebacterium species catalase?

A

Catalase positive

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18
Q

Corynebacterium species oxidase?

A

Oxidase negative

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19
Q

Corynebacterium species are said to resemble?

A

Chinese characters

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20
Q

Pathogenesis and pathogenicity of Corynebacterium species?

A

Opportunistic pyogenic bacteria that live in mucous membranes EXCEPT for Corynebacterium bovis

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21
Q

Pathogenesis and pathogenicity of Corynebacterium bovis?

A

Lives in the teat cistern of 20% of cows and protects the mammary gland against invasion by more virulent pathogens (causes mild neutrophil response)

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22
Q

Corynebacterium pseudotuberculosis ilness?

A

Caseous lymphadenitis or pseudotuberculosis. Mostly external lesions with onion ring appearance on cut section. One should be careful with pus because it is highly pathogenic.

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23
Q

Goats with pseudotuberculosis show?

A

Superficial abscesses

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24
Q

Transmission of pseudotuberculosis is via?

A

Herd transmission via pus discharges, nasal oral discharges, coughing, vectors

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25
Q

How contagious is pseudotuberculosis?

A

Extremely contagious

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26
Q

Incubation period of pseudotuberculosis

A

3 months

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27
Q

Corybacterium sampling?

A

Pus, exudate, samples of affected tissue, mid-stream urine

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28
Q

Culture of Corynebacterium?

A

Blood agar, O2, 24-48h, 37C

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29
Q

Rhodococcus equi appearance

A

Gram positve cocci or rods which are individually spread

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30
Q

Rhodococcus equi grows on?

A

Non enrichened media

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31
Q

Rhodococcus equi colony appearance?

A

Salmon-pink, mucoid, non-haemolytic colonies

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32
Q

Rhodococcus equi atmospheric requirements?

A

Aerobic

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33
Q

Rhodococcus equi motility?

A

non-motile

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34
Q

Rhodococcus equi lives?

A

As soil saprophyte also in digestive tract of horses

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35
Q

Rhodococcus equi is typically

A

A respiratory pathogen of foals (<6m)

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36
Q

Rhodococcus equi spreads?

A

From feces/environment as aerosol

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37
Q

Rhodococcus equi virulence factor

A

Specific superficial antigen

38
Q

Rhodococcus equi virulence depends on?

A

Temperature

in high t 34-41C lot of antigen production

39
Q

Rhodococcus equi causes?

A

Suppurative bronchopneumonia and abscessation in foals when t is high
Ocassionaly in pigs, cats and in immunosuppressed pple

40
Q

Rhodococcus equi lesions?

A

Nodules in lungs

41
Q

Rhodococcus equi diagnosis?

A

Leukocytosis

Lung auscultation and thorax x-ray

42
Q

Rhodococcus equi specimens?

A

Tracheal aspirates and pus from lesions (which need to be sent refrigerated)

43
Q

How is Rhodococcus equi cultured?

A

Aerobically in 37 C 24-48h in

  • nutrient agar
  • blood agar
  • macConkey
44
Q

How do catalase test results look like?

A

foamy if positive

clear/non-foamy if negative

45
Q

How do oxidase test results look like?

A

blue ink on strip if positive

clear paper if negative

46
Q

How is Rhodococcus equi in the oxidation-fermentation and sugar fermentation tests?

A

Unreactive

47
Q

No fermentation in the oxidation-fermentation and sugar fermentation tests?

A

Remains purple

48
Q

Fermentation with production of acid but no gas in the oxidation-fermentation and sugar fermentation tests?

A

Yellow colour

49
Q

Fermentation with production of acid and gas in the oxidation-fermentation and sugar fermentation tests?

A

Yellow colour and bubble

50
Q

Rhodococcus equi appearance in the CAMP test?

A

Arrowhead

51
Q

Actinobacteria is taxinomically a?

A

class

52
Q

Actinobacteria appearance

A

gram +

often branching filaments

53
Q

Actinobacteria as pathogens?

A

opportunistic

54
Q

Actinobacteria cause

A

inflammatory responses

55
Q

Actinobacteria species differenciation?

A

MZN staining

56
Q

MZN + appearance

A

red

57
Q

MZN - appearance

A

blue

58
Q

Actinomyces atmospheric conditions

A

anaerobic or facultatively anaerobic

59
Q

Actinomyces morphology

A

produce filaments, however morphologically heterogenous

60
Q

Actinomyces spore formation

A

no

61
Q

Actinomyces motility

A

no

62
Q

actinomyces MZN?

A

negative

63
Q

Actinomyces live in?

A

mucous membranes

64
Q

Most pathogenic actinomyces?

A

Actinomyces bovis

65
Q

Actinomyces bovis causes

A

Lumpy jaw disease

66
Q

Actinomyces bovis habitat

A

oropharynx

67
Q

Arcanobacterium pyogenes infects which species?

A

cattle, swine and sheep

68
Q

Arcanobacterium pyogenes habitat

A

Nasopharyngeal mucosa

69
Q

Actinobaculum suis habitat?

A

preputial mucosa

70
Q

Virulent factors of Arcanobacterium, Actinobaculum, and Actinomyces?

A

Haemolytic, dermonecrotic exotoxin
Proteases
Neuraminidases

71
Q

What kind of reactions Arcanobacterium causes?

A

Purulent reactions

72
Q

What kind of reactions Actinomyces causes?

A

Pyogranulomatous reactions

73
Q

Arcanobacterium pyogenes causes which illnesses?

A
Abscessation
mastitis
suppurative pneumonia
endometritis
pyometra
arthritis
umbilical infections
74
Q

Actinomyces bovis causes which illnesses?

A

Bovine actinomycosis = lumpy jaw

75
Q

Actinomyces viscosus causes which illnesses?

A

Canine actinomycosis:

  • cutaneous pyogranulomas
  • pyothorax and proliferative pyogranulomatous pleural lessions
  • disseminated lesions
76
Q

Actinobaculum suis causes

A

pyelonephritis

cystitis

77
Q

Which samples from Actinobaculum, Actinomyces and Arcanobacterium?

A

exudates, aspirates, tissue samples

78
Q

How to culture Actinobaculum, Actinomyces and Arcanobacterium?

A

Blood agar, anaerobiosi or aerobiosi 37C for 5 days

79
Q

How are Actinobaculum, Actinomyces and Arcanobacterium in catalase test?

A

Negative except Actinomyces viscosus

80
Q

What is special about Nocardia?

A

Spore formation

81
Q

Where do you cultivate Nocardia?

A

Sabouraud dextrose agar

82
Q

Nocardia MZN?

A

positive

83
Q

Nocardia lives in

A

Soil as a saprophyte

84
Q

Nocardia atmosphere requirements?

A

Aerobic

85
Q

How Nocardia infections occur?

A

Opportunistic bacteria
infections when immunosuppression
infection by teat canal, skin wounds, and inhalation

86
Q

Nocardia species infect

A

Dogs
Cattle
Pigs
sheep, goat and horses

87
Q

Nocardia species cause which conditions in dogs?

A

Canine nocardiosis:

  • cutaneous pyogranulomas
  • pyogranulomatous pleural lesions and pyothorax
  • disseminated lesions
88
Q

Nocardia species cause which conditions in cattle?

A

Chronic mastitis and abortions

89
Q

Nocardia species cause which conditions in pigs

A

Abortions

90
Q

Nocardia species cause which conditions in sheep, goats, and horses

A

Wound infections, mastitis, pneumonia, other pyogranulomatous conditions

91
Q

Nocardia farcinica causes what?

A

Bovine farcy