General bacteriology I Flashcards
Identification of pathogenic bacteria step 1
full clinical history
Identification of pathogenic bacteria step 2
Selection, collection and transportation of specimens
Identification of pathogenic bacteria step 3
Examination of stained smears
Identification of pathogenic bacteria step 4
Cultural and biochemical charasteristics
Identification of pathogenic bacteria step 5
Detection by immunological and molecular methods
Curve of bacterial growth
lag phase
exponential phase
stationary phase
decline phase
Factors which influence in vitro bacterial growth
nutritional genetic chemical physical environmental
Culture media
where the bacteria is grown in vitro
can be non enrichened or enrichened media
can be solid, semisolid or liquid media
nutrient agar
a basic medium where non-fastidious bacteria can grow.
Suitable for demonstrating bacterial morphology and pigment production, also used for viable counting methods.
Blood agar
An enrichened media which supports the growth of most pathogenic bacteria and is used for their primary isolation. It allows the recognition of bacterial hemolysin production.
MacConkey agar
A selective medium containing bile salts. Especially useful for isolation of enterobacteria and some other gram- bacteria. Allows differentiation of lactose fermenters and non-lactose fermenters.
Which color the lactose fermenters are in MacConkey agar?
Pink
Selenite or Rappaporth-Vassilidiasis broth
Selective enrichment media used for the isolation of Salmonellae from samples containing other gram- enteric organisms.
Edwards medium
A blood-agar-based selective medium used for the isolation and recognition of streptococci.
Chocolate agar
Heat-treated blood agar which supplies special growth requirements (X and V factors) for the isolation of Haemophilus species and for the culture of Taylorella equigenitalis.
