Glycolysis and TCA Cycle

Question 1

which tissue has hexokinase which has glucokinase

Answer 1

liver has glucokinas high km high vmax

all other tissue have hexokinase low vmax low km

Question 2

what regulates PFK-1

Answer 2

• citrate, ATP

+ AMP, F-2,6-BP

Question 3

what is the rate limiting step in glycolysis-

Answer 3

fructose 6P–> fructose 1,6 bisphoshpate

Question 4

what enzyme takes PEP to Pyruvate?

whats regulates it?

Answer 4

Pyruvate kinase

- glucagon

Question 5

What regenerates NAD+ in anaerobic conditions

Answer 5

the formation of lactate from pyruvate

Question 6

What takes Pyruvate to acetyl coa

how is it regulated

Answer 6

PDH complex, u lose a co2, gain an NADH per pyruvate

-NADH, Acetyl coa

Question 7

7. List the key intermediates of the TCA cycle and why they are important.

Answer 7

Oxaloacetate- binds with Acetylcoa to make citrate
citrate because it regulates Glycolysis
Isocitrate-
alpha ketoglutarate- amino acids can comei n here
succinyl coa- odd chain fatty acids can come in here
succinate- succinate to fumarate enzyme is complex 2 of electron transport chain and make FADH2
fumarate
malate

Question 8

8. List the principle products of the TCA cycle.

Answer 8

GTP, NADH, FADH2

Question 9

9. Describe the metabolic role of the TCA cycle

Answer 9

to make high energy intermediates in order to make ATP

Question 10

10. Describe how glycolysis and gluconeogenesis are coordinately regulated.

Answer 10

by insulin and glucagon. by high energy or low energy state

Question 11

what enzyme makes citrate-

Answer 11

citrate synthase

Question 12

what tissue have GLUT 2 transporter, which have Glut 4?

Answer 12

2- liver kidney brain, insulin independent

4- everywhere else, insulin dependent