Glycolysis

Question 1

Where does glycolysis occur?

Answer 1

In the cytosol

Question 2

What is the rate limiting enzyme?

Answer 2

Phosphofructokinase-1 (PFK-1)

Question 3

What is glycolysis stimulated by?

Answer 3

1. Insulin
2. AMP
3. Fructose-2,6-bisphosphate

Question 4

What is glycolysis inhibited by?

Answer 4

1. Glucagon
2. ATP
3. Citrate

Question 5

What are the 3 enzymes in the irreversible steps?

Answer 5

1. Hexokinase/glucokinase
2. PFK-1
3. Pyruvate kinase

Question 6

What are the products of glycolysis?

Answer 6

1. 2x ATP
2. 2x NADH
3. 2x pyruvate

Question 7

When is ATP used?

Answer 7

1. Conversion of glucose to G6P

2. Addition of phosphate to fructose-6-phosphate

Question 8

When is NADH and H+ produced and what is the enzyme involved?

Answer 8

Conversion of GAP to 1,3-bisphosphoglycerate (1,3 BPG)

Question 9

When is ATP produced?

Answer 9

1. Removal of phosphate from 1,3 BPG to 3-phosphoglycerate

2. Removal of phosphate from PEP to pyruvate

Question 10

Where is hexokinase found?

Answer 10

All tissues

Question 11

Where is glucokinase found?

Answer 11

1. Beta cells of pancreas

2. Liver tissue

Question 12

What is hexokinase stimulated by?

Answer 12

Insulin

Question 13

What is hexokinase inhibited by?

Answer 13

1. G6P

2. Glucagon

Question 14

What does hexokinase do?

Answer 14

Converts glucose to G6P

Question 15

What is PFK-1 stimulated by?

Answer 15

1. AMP
2. Fructose-2,6-bisphosphate
3. Insulin

Question 16

What is PFK-1 inhibited by?

Answer 16

1. ATP
2. Citrate
3. Low pH
4. Glucagon

Question 17

What does PFK-1 do?

Answer 17

Converts F6P into fructose-1,6-bisphosphate

Question 18

What is pyruvate kinase stimulated by?

Answer 18

Fructose-1,6-bisphosphate

Question 19

What is pyruvate kinase inhibited by?

Answer 19

1. ATP

2. Alanine

Question 20

What does pyruvate kinase do?

Answer 20

Converts PEP to pyruvate