Glycolysis Flashcards
List enzymes in order in priming phase of glycolysis
Hexokinase
Phosphohexase isomerase
Phosphofructokinase
Aldose
Hexokinase
Phosphorylates C6
Phosphohexase isomerase
Moves carbonyl (double bond) from C1 to C2
Phosphofructokinase
Phosphorylates C1
Aldose
Cleaves after C3–>2 PGALs
List enzymes in payoff phase of glycolysis
Dehydrogenase Kinase Mutase Enolase Pyruvate kinase
Dehydrogenase
C3 is phosphorylated
Kinase
Substrate level phosphorylation–> ATP
Mutase
Phosphoryl moves from C3 to C2
Enolase
Dehydration (double bond between C1=C2)
Pyruvate kinase
Substrate level phosphorylation—>ATP
1 Glucose yields…
2 pyruvate
2 NADH
2 ADP
4 ATP (2 net)
How many CO2 produced in glycolysis
None
Why is G6P isomerase drop F6P
Allows phosphorylation at C1 amd that both sides have a P
F16BP to two pyruvate yields
2 NADH
4 ATP
How would the rxn of F16BP to pyruvate run spontaneously to the right
Have a higher conc of F16BP than product
If C1 in glucose is labeled, what in pyruvate are labeled
Methyl carbon
Which reactions I’m glycolysis require ATP
Hexokinase
Phosphofructokinase
Which rxn produces ATP as a product
Pyruvate kinase
Kinase
