Global Control

Question 1

Global control in bacteria

Answer 1

Control above operon level

Question 2

Regulon

Answer 2

Set of operons controlled by common regulator

Question 3

Modulon

Answer 3

operons that are subject by common regulator despite being part of different regulons

Question 4

Stimulon

Answer 4

Collection of genes/operons that all responsd to particular environmental stimulus

Question 5

Identification methods of global control networks

Answer 5

1. Biochemical (proteomics)
2. Genetic (gene fusion)
3. Transcriptomics

Question 6

Operon

Answer 6

Group of related genes with a single promoter

Question 7

Ferric Iron uptake regulator

Answer 7

Example of a modulon
Transcription that represses siderophore synthesis

Question 8

isoelectric point

Answer 8

the pH at which the net charge of a protein molecule is zero

Question 9

2D gel electrophoresis

Answer 9

1st dimension - molecules are separated linearly according to their isoelectric point.

2nd dimension - the molecules are then separated at 90 degrees from the first electropherogram according to molecular mass.

SDS-PAGE

Question 10

Analysis of protein spot

Answer 10

Cut out gel
Trypsin digestion to generate n-peptides
Mass spectrometry

Question 11

Mass Spectrometry

Answer 11

Analytical tool useful for measuring the mass-to-charge ratio (m/z) of one or more molecules present in a sample

Question 12

iTRAQ

Answer 12

Isobaric tag for relative and absolute quantification

Question 13

Isobaric

Answer 13

Identical mass

Question 14

Transcriptomics

Answer 14

Investigate alternatively spliced transcripts
RNA-SEQ

Question 15

Gene fusion using LacZ