glasswares Flashcards
1
Q
labware can either be
A
glass or plastic
2
Q
they are more expensive and requires care while cleaning to prevent breakage and scratches
A
glass containers
3
Q
these containers are not reusable
A
plasticwares
4
Q
labwares are required to be _____ to eliminate the possibility of contamination of solution due to inadequate cleansing
A
disposable
5
Q
what are the types of glasswares
A
borosilicate glass (pyrex/kimax)
boron free glassware/ soft glass
corex ( corning)
flint glass
vycor
6
Q
used for heating and sterilization processes
A
borosilicate glass
7
Q
characterized by high degreeof thermal resistance
A
borosilicate glass
8
Q
high resistance to alkaline
A
boron-free glassware
9
Q
thermal resistance is less compared to borosilicate glass
A
boron-free glassware
10
Q
made up of alumina-silicate glass that has been strengthened chemically and thermally
A
corex
11
Q
six times stronger compared to borosilicate glass
A
corex
12
Q
made up of soda-lime and a mixture of calcium, silicon, sodium oxides
A
flint glass
13
Q
poor resistance to high temperature
A
flint glass
14
Q
for solutions that need heating at a high temperature
A
vycor
15
Q
can be heated to 900 degrees and can be used for extreme high temperature
A
vycor
16
Q
what are the types of plasticware
A
polyolefins/polyethylene/ polypropylene
polycarbonate
fluorocarbon resins or teflon
17
Q
chemically inert resins
A
polyolefins
18
Q
resistant to acids, alkalis, salt solutions
A
polyolefins
19
Q
has a clear appearance and because of its strength it may be used for centrifuge tube
A
polycarbonate
20
Q
chemical resistance is less than polyolefins
A
polycarbonate
21
Q
chemically inert and used for high temperature from -270 degree celcius to +255 degree celcius
A
fluorocarbon resins or teflon
22
Q
provides non-wettable surface
A
fluorocarbon resins or teflon
23
Q
used for stir bars and tubing
A
fluorocarbon resins or teflon
24
Q
what are the common glasswares in the lab?
A
test tubes
pipette
micropipette
automatic pipette
petri dish
beakers
flasks
stirring rod
graduated cylinder
vials
25
most common glassware
test tube
26
also known as the culture tube (microbiology section) or sample tube
test tube
27
common piece of glassware consisting of a finger-like length of a glass or clear plastic tubing, open at the top and closed at the bottom
test tube
28
used as a container for mixing the specimen of a patient
test tube
29
are glass or plastic tubes that are usually open at both ends
pipettes
30
used to transfer SPECIFIC AMOUNT of liquid from one container to another
pipette
31
used for volumes from 1 to 100 millimeters
pipette
32
one of the skills that medtech should developed inside the laboratory
pipetting
33
what are the classifications for pipet
calibration marks or design
drainage characteristics
type
34
under calibration marks or design
to deliver (TD)
to contain (TC)
35
under drainage characteristics
blow-out (TC)
self-drainage (TD)
36
under type
transfer
graduated or measuring pipet
37
where is calibration mark seen?
on the body of the pipette
38
it DELIVERS THE EXACT AMOUNT it hold into the container
to deliver
39
it HOLDS THE PARTICULAR VOLUME but does not dispense the exact volume
to contain
40
it has a continuous frosted rings (colored rings) on top of the pipet
blowout (TC)
41
exact volume is obtained when the last drop is blown out using an aspirator
blowout
42
absence of frosted rings and liquid can be drain by gravity
self draining (TD)
43
kinds of transfer type
volumetric pipet
ostwald folin
van slyke pipet
pasteur pipet
44
for non-viscous fluid; self-draining; small amount left in the tip should not be blown out; used to deliver a specific volume of liquid
volumetric pipet
45
shaped like rolling pins with a large belly, one blunt end (neck), one tapering end
volumetric pipet
46
for viscous fluid; with etched rings; bulb closer to the delivery tip; these are blown out pipets
ostwald folin
47
bulb is situated between the mouthpiece and the delivery tip
van slyke pipets
48
transfer fluid without consideration of a specific volume
pasteur pipet
49
don't pay much to graduation marks
pasteur pipets
50
types of graduated or measuring pipet
serological pipet and mohr pipet
51
with graduation up to the tip; blowout pipet
serological pipet
52
without graduation to the tip; calibrated between 2 marks; self draining
mohr pipet
53
if the delivery tip is not calibrated up to the 10th mark it is considered a
serological pipet
54
if calibration to 10 ml, it is a
mohr pipet
55
If a frosted band or two thin rings around the
neck, then it is a
blowout pipet
56
these are the manufacturers code for the maximum volume of the pipet
thick colored rings or colored dots
57
for quantities less than 1 mL or 1000 microliters; to contain pipets
micropipettes
58
examples of micropipettes
rbc and wbc thoma pipets
sahli-hellige pipets
lang-levy pipets
59
used for MANUAL CELL COUNTS in the hematology section
rbc and wbc thoma pipets
60
manual determination of hemoglobin
sahli-hellige pipets
61
the 2 kinds of thomas pipettes
rbc pipette and wbc pipette
62
same parts of rbc and wbc pipette
calibrated stem
bulb
short stem
63
differences between rbc and wbc pipette
bead color
graduation mark
size of the bulb
size of the lumen
64
bead color for rbc thoma pipette
red
65
bead color for wbc thoma pipette
white
66
graduation mark for rbc thoma pipette
graduations up to 101 mark
67
graduation mark for wbc thoma pipette
graduations up to 11 mark
68
size of the bulb for rbc thoma pipette
larger
69
size of the bulb for wbc thoma pipette
smaller
70
size of the lumen for rbc thoma pipette
smaller
71
size of the lumen for wbc thoma pipette
larger
72
more accurate and precise when it comes in aspirating and dispensing the solution into another container
automatic pipettes
73
type of automatic pipettes
handheld and automated
74
uses disposable tips and the technologist performs aspiration and dispensing
handheld
75
types of automated pipettes
air displacement pipette, positive displacement pipette , dispenser or diluter pipette
76
it RELIES TO THE PISTON FOR SUCTION CREATION to draw the sample into a disposable tip
air displacement pipet
77
the piston does not come in contact with the liquid
air displacement pipet
78
factors that can affect the performance of the air displacement pipet
temperature
atmospheric pressure
gravity
79
how does the air displacement pipet works
1. the piston moves to the appropriate position when the volume is set
2. when the operating button is pressed to the first stop, the piston expels the same volume of air as indicated on the volume setting
3. after immersing the tip into the liquid, the operating button is released. this will signal for the pipet to aspirate the sample. this creates a partial vacuum, and the specified volume of liquid is aspirated into the tip.
4. when the operating button is pressed at the first stop again, the air dispenses the liquid. to completely empty the tip, press the operating button to the second stop
80
parts of the air displacement pipet
piston
shaft
air space
disposable tip
81
it operates by moving the piston in the pipet tip or barrel, much like a hypodermic syringe
positive displacement pipet
82
the piston touches the sample and do not require different tip for each use
positive displacement pipet
83
the tip of the piston is disposable
positive displacement pipet
84
based on direct contact of the piston with the liquid or sample. the aspirated liquid amount
depends on the
dimension of the cylinder or capillary and the movement distance of the piston
85
how does the positive displacement pipet work?
1. the piston moves down inside the tip to make direct contact with the sample
2. the piston moves up to draw the sample into the tip
3. when dispensing, the piston descends, and the selected volume is dispensed
86
it obtains liquid from a common reservoir and dispensed repeatedly
dispenser or diluter pipet
87
combines sampling and dispensing functions
dispenser or diluter pipet
88
parts of a positive displacement pipet
shaft
disposable piston
disposable capillary
piston seal
89
they are made primarily of polypropylene
plastic tips
90
as the fluid is allowed to drain at the receiving vessel, the pipet should be held in a _________ position with the tip against the side of the receptacle
slanted
91
imperfect wetting or the presence of discreet droplet of water indicates that the pipette is not
sufficiently clean
92
when we used automatic pipette with tips, make sure that there is no ________ present inside the tip or plastic tip
bubbles or spaces
93
petri dish was invented by _______ in _____
Julius Richard Petri in 1887
94
simple pair of nesting glass dishes - keeping specimens of growing bacteria sterile
petri dish
95
shallow cylindrical containers with fitted lids that are designed specifically for microbiology or culture cell use
petri dish
96
typically made of borosilicate glass or clear plastic (usually polystyrene or polycarbonate) and come in variety of sizes
petri dish
97
petri dish can't be reusable or disposable
false
98
what is the procedure for glass petri dish?
autoclave, remove agar, clean the dish, put it back in the autoclave for sterilization
99
what is the procedure for plastic petri dish?
autoclave then discard
100
these beakers can be heated to a higher temperature compared to plastic counterparts, while offering HIGHER CLARITY for CONTENT VISIBILITY and MEASURING
glass beakers
101
features of glass beakers
uniform sidewalls, bottom thickness with a flat bottom and a beak, or small spout, for easier pouring
102
used for precise volume measurement
glass beakers
103
they have _____ on their side that are accurate within 10%
graduated markings
104
what are the other term for Erlenmeyer flask
also known as conical flask or titration flask
105
features of an erlenmeyer flask
flat bottom
conical body
cylindrical neck
106
they have wide bases with sides that taper upwards to a short vertical neck
erlenmeyer flask
107
allow the contents of the flask to be mixed by swirling without the risk of spilling, making it suitable for titration
slanted size and narrow neck
108
suitable for boiling liquid
erlenmeyer flask
109
what is the other term for volumetric flask
measuring flask or graduated flask
110
features of a volumetric flask
pear-shaped with a flat bottom and made up of glass or plastic
111
they are _____ to contain a precise volume at a certain temperature used for precise dilutions or preparations of standard solution
calibrated
112
also known as boiling flask
florence flask
113
features of florence flask
round body
long neck
often a flat bottom ( sometimes produced with a round bottom and thus need support in order to stand upright)
114
designed for uniform heating, distillation, and ease of swirling
often made of borosilicate glass to resist high temp
designed for chemical resistance
florence flask
115
also known as the glass rod
stirring rod
116
what are the types of flask
erlenmeyer flask
volumetric flask
florence flask
117
use of stirring rod
mix chemicals and decanting supernatants
118
stirring rod is made of
borosilicate glass or polypropylene plastic
119
usually made of solid glass, about the thickness and slightly longer than a drinking straw, with rounded edges
stirring rod or glass rod
120
why is the edge of the stirring rod smooth and rounded?
to prevent scratching the surface of glassware during use which may lead to crack when heated
121
also known as the measuring cylinder or mixing cylinder
graduated cylinder
122
the graduated cylinder measures the what?
volume of a liquid
123
features of a graduated cylinder
narrow cylindrical shape with each marked line representing the amount of liquid that has been measured
124
graduated cylinder is usually made up of ?
polypropylene for excellent chemical resistance
polymethyl pentene - for transparency
making them lighter and less fragile than glass
125
traditional graduated cylinder is usually narrow and tall to
increase the accuracy and precision
of volume measurements
126
is graduated cylinder appropriate to be used in volumetric analysis?
nauurrr
127
used for general collection, storage, transportation of samples
vials
128
may be sterile, non-sterile, and/or autoclavable
vials
