Genetically Engineered Animals 10

Question 1

What is a transgenic animal?

Answer 1

additional genes are introduced

Question 2

How are transgenic mice created?

Answer 2

Fertilised egg prior to
fusion of the pronuclei. 
Use microinjection to 
insert foreign DNA.
Eggs transferred into 
foster mother
Analyse offspring for presence
of transgene (PCR)
Breed positive mice to
generate homozygous mice

Question 3

Give 3 uses of transgenic animals

Answer 3

Model of disease
Insect control
Alter characteristics

Question 4

What is the chord mouse experiment?

Answer 4

Transgenic mouse expressing green fluorescent

Question 5

What are knockout mice?

Answer 5

In these animals a wild type gene is replaced with an inactive gene to create a “gene knockout”.

Question 6

What is the difference between transgenic and knockout?

Answer 6

T: Exogenous DNA inserted into random non-homologous site
K: Insertion of modified DNA at a specific homologous site

Question 7

How are knockout mice created?

Answer 7

Culture of ES cells