Gene Expression 3

Question 1

Is mRNA stable?

Answer 1

No

Question 2

What are 3 techniques for measuring mRNA levels?

Answer 2

Northern blotting

RT-PCR

Question 3

What technique is used to measure protein levels?

Answer 3

Western blotting

Question 4

Describe the process of northern blotting

Answer 4

RNA subject to electrophoresis

Use of intracellular membrane

Question 5

What does Northern blotting reveal?

Answer 5

mRNA size
Tissue
Specific Expression
Kinetic pattern of expression

Question 6

What does RT PCR stand for?

Answer 6

Reverse transcriptase polymerase chain reaction

Question 7

What is the process for PT PCR?

Answer 7

cDNA synthesis
PCR amplification
Gel electrophoresis

Question 8

What does PT PCR measure?

Answer 8

Amount of product at each PCR cycle

Question 9

What is a microarray?

Answer 9

Set of DNA fragments attached to a glass microscope slide

Question 10

What is a gene chip?

Answer 10

Collection of DNA oligonucleotides on a stamp sized chip

Question 11

What do microarrays and gene chips compare?

Answer 11

Patterns of gene expression in different tissues

Question 12

What does a result of no colour mean when using microarrays and gene chips?

Answer 12

The gene is not expressed

Question 13

What technique is used for whole transciptome analysis?

Answer 13

Microarrays

Question 14

What is epigenetic?

Answer 14

Heritable changes in gene activity thats are not result of changes in DNA sequencing