genetic information, variation and relationships between organisms Flashcards
prokaryotic cell DNA
- short
- circular
- not associated with histones
eukaroytic cell DNA
- long
- linear
- associated with histones
proteins associated with eukaryotic DNA
histones
gene
base sequence of DNA that codes for an amino acid in a polypeptide or a functional RNA
fixed position of a gene on a particular DNA molcule
locus
triplet
sequence of three DNA bases
role of triplet
codes for a specific amino acid
genetic code
non-overlapping, universal and degenerate
non-overlapping code
each base is read as a discrete unit of 3
universal code
the same codons code for the same amino acids in all organisms
degenerate code
each amino acid is coded for by more than one triplet of bases
exons
coding areas of DNA
introns
non-coding areas of DNA only found in eukaryotes between exons in genes
splicing
introns are removed from pre-mRNA and exons are joined together
genome
complete set of genes in a cell
proteome
full range of proteins that a cell is able to produce
mRNA
messenger RNA
tRNA
transfer RNA
structure of mRNA
- short
- single stranded
- codons
structure of tRNA
- three bases called anticodon
- amino acid binding site
- clover shaped formed from hydrogen bonds
transcription
production of mRNA from DNA
process of transcription
- DNA helicase unwinds comp. base paires between DNA helix
- one strand acts as a template
- free mRNA molecules in nucleus align by comp. base pairing (A-U and C-G)
- RNA polymerase catalyses condensation reaction between adjacent RNA nucleotides
- creating PPD bonds
- pre-mRNA can then be sliced
RNA polymerase
catalyse condensation reaction between adjacent RNA nucleotides to form phosphodiester bonds
prokaryote transcription
transcription results directly in the production
of mRNA from DNA
eukaryote transcription
transcription results in the production of pre-mRNA; this is then spliced to form mRNA
translation
production of polypeptides from the sequence of codons carried by mRNA
process of translation
- mRNA attaches to ribosome
- tRNA anticodons bind to complementary mRNA codons
- tRNA brings specific amino acids
- amino acids form peptide bonds with the use of ATP
- tRNA is then released
- ribosome moves along mRNA to form polypeptide
ribosome structure
rRNA and protein
rRNA
ribosomal RNA
gene mutation
change in the base sequence of DNA