energy transfers in and between organisms Flashcards
where does LDR take place?
thylakoid membrane
where does LIR take place?
stroma
stages of LDR
- photolysis
- photoionisation of chlorophyll
- chemiosmosis
- production of ATP and NADPH
photolysis of water
- light energy is abosrbed by chlorophyll
- H2O -> 1/2O2 + 2e- + 2H+
- H+ is used to make NADPH
- e- passed along electron transport chain
- O2 used for respirartion
photoionisation of chlorophyll
light energy absorbed by chlorophyll excites electrons, raising them to a higher energy level, so they leave the chlorophyll
chemiosmosis/ETC in photosynthesis
- electrons move along photosystems
- electrons release energy and some is used to actively transport protons across chloroplast membrane into thylakoid space
- creates electrochemical gradient
- protons pass through ATP synthase which phosphorylates ADP into ATP
- protons phosphorylate NADP to become NADPH
LIR/calvin cycle
- carbon fixation (1c) with RuBP (5c) to form two molecules of GP (3c)
- this is catalysed by the enzyme RuBisCo
- GP is reduced into triose phosphate using 2ATP and oxidising 2NADPH
- 1/6 of triose phosphate carbon is used to make a hexose sugar
- rest of triose phosphate is used to regenerate RuBP using ATP
how many times must the calvin cycle happen to create a hexose sugar?
6
how does structure of chloroplast maximise the rate of LDR?
- ATP synthase channels within granal membrane
- large surface area of thylakoids
- photosystems position chlorophyll for maximum light absorption
how does structure of chloroplast maximise the rate of LIR?
- own DNA and ribosomes for synthesis of enzymes
- concentrations of enzymes and substrates in stroma is high
what is a limiting factor?
factor that can reduce the rate of photosynthesis, determining its maximum rate
limiting factors of photosynthesis
temperature, light intensity, carbon dioxide concentration
why is temperature a limiting factor of photosynthesis?
LIR is an enzyme controlled process, thus tempertaure increases rate until it becomes too high and denatures enzymes and no E-S complexes will be made
why is light intensity a limiting factor of photosynthesis?
light energy is needed in LDR for photolysis and photoionisation
why is carbon dioxide a limiting factor of photosynthesis?
carbon fixation in LIR
argicultural practices to remove limiting factors
- artificial light
- artifical heat
- addition of CO2
stages of aerobic respiration
- glycolysis
- link reaction
- krebs cycle
- oxidative phosphorlyation
process of glycolysis
- phosphorylation of glucose to make glucose phospahte, using ATP
- production of triose phosphate
- oxidation of triose phosphate to produce pyruvate using 2ATP and 2NADH
where does glycolysis occur?
cytoplasm
products of glycolysis
- 2 pyruvate
- net gain of 2 ATP
- 2 NADH
process of link reaction
- pryvuate and NADH are actively transported from cytoplasm into mitochondrial matrix
- pyruvate is oxidised into acetate, losing one molecule of carbon
- NAD is reduced
- acetate combines with coenzyme A to produce acetylcoenzyme A
where does the link reaction occur?
mitochondrial matrix
products of link reaction
- 2 acetyl CoA
- 2 CO2 released
- 2 NADH
process of krebs/citric acid cycle
- acetyl CoA reacts with oxaloacetic acid (4c), releaseing coenzyme A and producing citric acid (6c)
- in a series of redox reaction, krebs generates NADH, FADH, ATP (by substrate level phosphorlylation) and loses CO2
where does krebs occur?
mitochondiral matrix
products of krebs
per glucose
- 6 NADH
- 2 FADH
- 2 ATP
- 4CO2
process of oxidative phosphorlylation/electron transport chain
- H+ from coenzymes are oxidised into protons and electrons
- electrons pass through ETC
- when they pass through proteins they release energy for protons to move into intermembrane space
- this creates an electrochemical gradient where there is a high conc. of protons in IMS
- protons move down conc. gradient through ATP synthase by facilitated diffusion
- this phosphorylates ADP into ATP
- oxygen is the final electron acceptor
- oxygen picks up protons to create water in respiration
what two molecules can be used as alternative respiratory substrates?
amino acids from proteins
glycerol and fatty acids from lipids
lipids as a respiratory substrate
phosphorylation of glycerol = triose phosphate
fatty acid = acetate
proteins as a respiratory substrate
deamination produces 3c compounds (pyruvate) and 4c/5c compounds (krebs)