Genetic engineering and biotechnology Flashcards
Brings to mind the work of designing and creating large structures such as roads and bridges.
Engineering
The process of modifying the genes of selected organisms to create genetically modified organisms.
Genetic engineering
An extrachromosomal circular DNA strand in the bacterium’s cytoplasm
Plasmid
This bacterium causes crown gall disease in plants
Agrobacterium tumefaciens
This bacterium has been chosen because of the lethal protein called the Bt toxin that it produces.
Bacillus thuringiensis
Also known as DNA-cutting enzymes because their main roles is to cleave the DNA into fragments on specific recognition sites.
Restriction enzymes
Sequences that when spelled backwards is the same as the word when spelled forward.
Palindromes
A DNA-joining enzyme because its main role is to ligate or join the two DNA strands.
DNA ligase
A natural process where bacteria can take up the genetic material in the environment.
Transformation
It is called the __________ if the bacterial host cell ingests a plasmid
Transformed cells
Those that take up the recombinant plasmid with the gene of interest.
Recombinant cells
Those that ingest a plasmid but without the gene of interest.
Nonrecombinant cells
Those that did not take up any plasmid
Nontransformed cells
A medium with antibiotic where transformed and non-transformed cells are placed.
Ampicillin
Ensures the growth of transformed cells.
The fourth step: Cloning