genetic engineering 2

Question 1

give 3 purposes of DNA sequencing?

Answer 1

predict function of DNA sequence
to confirm genetic sequence of engineered DNA
to identify between normal and mutated genes

Question 2

how many DNA bases must be known to make a primer?

Answer 2

15

Question 3

what mothod is used to sequence DNA?

Answer 3

sanger method (chain termination), then gel electrophoreses

Question 4

what do terminator nucleotides do and why?

Answer 4

prevent addition of further nucleotides/bases

do this as there is no OH at carbon 3 (only H)

Question 5

how many times is DNA sequenced in deep sequencing?

Answer 5

7

Question 6

what is required for sanger method?

Answer 6

DNA template, primer, DNA polymerase, free/normal bases, terminator bases(labelled)

Question 7

what are restriction enxymes?

Answer 7

precise DNA cutters. they recognise and cleave DNA at specific sites sequences

Question 8

what do restriction enzymes come from?

Answer 8

bacteria as protect bacteria from bacteriophages

Question 9

how does bacteria protect own DNA from restriction enzymes?

Answer 9

methylation of own restriction sites

Question 10

where is ecoR1 isolated from?

Answer 10

escherichia coli

Question 11

giev 2 characteristsics of recognition sites

Answer 11

are paladromic (read same forward and backwward on sense and antisense)
are short 4-8 bps