General Flashcards

1
Q

what are the two forms of reticulocytes in cats? How long do they last?

A

aggregate- 12hr-4-7d punctate- 10-12days

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2
Q

What species is more likely to get oxidation of Hb?

A

Cats- have more sulfahydryl groups that are targets for oxidation

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3
Q

In what breed of dog is von Willebrand’s deficiency common?

A

Dobermans

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4
Q

In which species is it normal to see small amount of heinz bodies?

A

cats

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5
Q

In which species are Howell-Jolly bodies normal?

A

horses & cats

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6
Q

In dogs, lithium heparin can overestimate which value?

A

albumin

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7
Q

What is greater in plasma vs. serum

A

more in serum- K, P, LDH (AST and CK in cattle/sheep

more in plasma- total proteins, globulins

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8
Q

What are the three things we need to be cautious about when using EDTA in terms of blood values?

A
  1. more K
  2. chelate divalent ions- Ca & Mg and effect enzymes (ALP, CK, glucose, amylase, lipase)
  3. acidosis (bicarbonate low)
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9
Q

On blood work, I see an increased glucose and diluted electrolytes- what does that indicate?

A

dextrose solution contamination

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10
Q

What are the three things that will happen with old blood?

A

cell lysis

RBC swelling (MCV, MCHC, PCV)

Platelet activation (MPV & platelet count)

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11
Q

What does long term storage do to blood?

A

decrease glucose

increase K as leaks from cells (horses, most LA, japenese dogs)

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12
Q

What is the corrected WBC count formula?

A

WBC x 100/ 100 + nRBC/100 WBC

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13
Q

Exposure of blood to light will decrease what values?

A

bilirubin

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14
Q

What analysis type is better at dealing with icterus & hemolysis?

A

reflectance chemistry

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15
Q

What are the things that affect absorbance?

A

lipolysis

hemolysis

icterus

hyperproteinemia

drug-KBr (hperhloremia)

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16
Q

What is the abnormal slide due to?

A
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17
Q

WBC count calculation?

A

Avg #/100x x 10,000 (or square of objective)= cell/uL in 10 fields

18
Q

In what animals are large platelets norma?

A

cats, Cavalier king charles spaniel

19
Q

What is the min # platelets/100x field normal?

A

dog and cats- 7-10 platelets/100x hpf

20
Q

IF a horse of ruminant has pale-yellow plasma, what does that indicate?

A

normal- from caretenoids in diet

21
Q

What is it?

A

reactive lymphocyte

22
Q

With lipemia, what values are affect?

A

decrease electrolytes

increased Hb

23
Q

What is the formula for Hct? What can falsely decrease it?

A

Hct= MCV x RBC conc/10

autoagglutination

24
Q

Formula for MCHC

A

Hb/PCV x 100= g/dL

25
Q

What are the things that could cause hyperchromasia?

A

ALWAYS ARTIFACT

hemolysis of sample

interference of Hb measure (lipemia, Heinz body, extreme WBC elevation)

26
Q

Will we see reticulocytosis in horses?

A

no

27
Q

Formula for absolute reticulocyte count?

A

RBC ct/uL x uncorrected reticulocyte ct in decimal

28
Q

FOrmular for corrected reticulocyte percent?

A

PCV/mean norm. PCV for species x uncorrected reticulocyte ct in %= %

29
Q

How soon will a low PCV and panhypoproteinemia be observed after blood loss?

A

12-24hrs

30
Q

what are three things that can affect my PCV acutely?

A

splenic cx

autotransfusion (slower)

administration of fluids

31
Q

How can you tell the difference between intravascular and extravascular hemolysis?

A

intravascular- Hbnemia/uria (haptoglobin overwhelmed)

32
Q

For hemolysis, what is the order of things you will see w/ bilirubin

A
  1. bilirubinuria
  2. bilirubinemia
  3. icterus (>2g/dL)
33
Q

What are these cells? What do they indicate?

A

acanthocytes

liver dz

fragmentation hemolysis

lipid disorders

34
Q

What are the three types of fragmentation?

A

mechanical injury (DIC, heartworms)

endothelial (vasculitis)

thermal (heatstroke)

35
Q

What are the cells? What do they indicate?

A

echinocytes (crenation)

old blood, EDTA, snak bites

36
Q

What are the cells? What do they indicate?

A

schistocytes

fragmentation, DIC

37
Q

What are the cells? What do they indicate?

A

spherocytes

immune-mediated hemolysis

on edge of blood film

fragmentation/blood transfusion

38
Q

What are the cells? What do they indicate?

A

RBC ghosts

intravascular immune hemolysis

39
Q

What cells are they? What do they indicate?

A

eccentrocytes anad pyknocyte

oxidative damage

40
Q

How to perform saline Test?

A

1 drop EDTA blood: 9 drops saline

mix and mount

41
Q

If the coomb’s test is negative, can I rule out immune mediated hemolysis?

A

NO

42
Q

What are potential causes of hyperchromasia?

A

hemolysis

lipemia

heinz bodies

extreme WBC elevation