gene & SC therapy

Question 1

pre-implantation genetic diagnosis

Answer 1

genetic profiling of embryos before implantation
isolation of DNA from cultures embryo - remove cell(s) for testing and culture rest of embryo normally (okay as regulative development)

cell source options -
- polar bodies
- cleavage stage embryo
- trophectoderm of blastocyst

PCR & sequencing
fluorescence in situ hybridisation
whole genome hybridisation, or arrays

Question 2

strategies for getting genes into cells

Answer 2

viral delivery
chemical delivery - liposomes, nanoparticles, naked DNA