Gene Expression Flashcards
acetylation
The addition of acetyl groups to histones. Acetylation activates the gene by
making it more accessible to transcription factors.
addition
A form of gene mutation in which one or more nucleotide bases are inserted into a DNA sequence. This may result in a frameshift to the right.
benign
Describes a tumour that is non-cancerous. Such tumours grow slowly, are enclosed in a capsule and remain at the site of origin. They can usually be removed by surgery.
cancer
a non-communicable disease resulting from tumour cells that metastasise
cellular proteome
the proteins expressed in a given type of cell
complementary DNA (cDNA)
A single strand of DNA complementary to the mRNA template strand.
complete proteome
all of the proteins coded for by the genome
deletion
A form of gene mutation in which one or more nucleotide bases are removed from
a DNA sequence. This may result in a frameshift to the left.
differentiation
a process in which cells become specialised for function
DNA hybridisation
The process by which a single-stranded segment of DNA is combined with a complementary fragment of DNA or RNA.
DNA ligase
An enzyme that joins the sugar-phosphate backbone of two DNA segments.
DNA polymerase
An enzyme that synthesises a double-stranded molecule of DNA from a
single template strand using complementary nucleotides.
DNA probe
A short, single-stranded segment of DNA that can be fluorescently or radioactively labelled. DNA probes are used to locate specific alleles of genes.
DNA sequencing
Determining the entire DNA nucleotide base sequence of an organism.
duplication
A form of gene mutation in which one or more nucleotide bases are repeated.
This may result in a frameshift to the right.
epigenetics
The study of changes in gene expression that are not due to alterations in the nucleotide base sequence of DNA.
frameshift mutation
A form of gene mutation in which the addition or deletion of nucleotide bases alters all subsequent triplet codes in a DNA sequence. This often leads to the production of a non-functional protein.
gel electrophoresis
A technique that separates fragments of DNA by size using electric current.
gene machine
A method of artificially manufacturing genes by feeding the desired sequence of bases into a computer.
gene mutation
A change to at least one nucleotide base in DNA or the arrangement of bases. Gene mutations occur spontaneously and may result in changes to genotype.
gene therapy
A technique in which a functional gene, cloned from a healthy individual, is inserted into cells that lack the gene.
genetically modified organism (GMO)
an organism that has had its genome altered
genetic counselling
A service that provides information and advice to people affected by
or at risk of genetic diseases. This helps individuals and families to make informed decisions.
genetic fingerprinting
A technique used to genetically identify an organism. It has applications in forensics, paternity testing, diagnostics and the breeding of plants and animals.
genetic screening
testing individuals for certain faulty alleles
genome
the complete genetic material of an organism
hypermethylation
Increased methylation of DNA. This results in the inactivation of tumour suppressor genes and the resulting formation of tumours.
hypomethylation
Reduced methylation of DNA. This results in the activation of oncogenes genes and the resulting formation of tumours.
induced pluripotent stem (iPS) cells
Unipotent cells that have been reprogrammed (using transcriptional factors) to become pluripotent stem cells. iPS cells are capable of self-renewal.