Gene Expression Flashcards
LTP
synapse strengthening in response to activity
new RNA synthesis is required for what stage of LTP?
later stage
protein synthesis is required for what stage of LTP?
Later stage
how many genes encode for proteins?
20,000 to 25,000
how does DNA differ from RNA
DNA missing hydroxy group, thymine
which techniques utilise mRNA probes
in situ hybridisation
RT-PCR
northern blot
which techniques utilise antibodoes to identify proteins?
western blot
immunihistochemistry
immunocytochemistry
where does alpha bungarotoxin bind to?
α7 subunit of nicotinic acetylcholine receptor
location of α7 subunit of nicotinic acetylcholine receptor
cortex
which enzyme carried out transcription?
RNA polymerase
alternative splicing occurs to…
mature mRNA
introns are removed from…
immature mRNA
properties of mature mRNA
5’ cap and polyA tail
Steps involved in cloning
- RNA isolation and fractionation
- mRNA to produce cDNA
- cDNA library construction
how is gene function determined?
electrophysiology
Expression cloning
plasmids used generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest are recovered for further analysis
mammalian cloning techniques
Expression cloning
DNA probe
low stringency hybridisation
ansiomycin
inhibits protein synthesis
actinomysin D
inhibits RNA synthesis
transcription factors can be either
promoters are shared by
multiple genes
the transcription factors TF2D and TBP bind to which promoter?
TATA
what does TF2D recuit?
TF2B
what does TB2B recruit?
TF2F and RNApol 2
How do TF2E and TF2H activate transcription?
contain kinases which phosphorylate C tail of RNA pol
repressors and enhancers
DNA sequences affecting transcription
