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Molecular Neuroscience > Gene Expression > Flashcards

Gene Expression Flashcards

1
Q

LTP

A

synapse strengthening in response to activity

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2
Q

new RNA synthesis is required for what stage of LTP?

A

later stage

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3
Q

protein synthesis is required for what stage of LTP?

A

Later stage

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4
Q

how many genes encode for proteins?

A

20,000 to 25,000

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5
Q

how does DNA differ from RNA

A

DNA missing hydroxy group, thymine

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6
Q

which techniques utilise mRNA probes

A

in situ hybridisation
RT-PCR
northern blot

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7
Q

which techniques utilise antibodoes to identify proteins?

A

western blot
immunihistochemistry
immunocytochemistry

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8
Q

where does alpha bungarotoxin bind to?

A

α7 subunit of nicotinic acetylcholine receptor

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9
Q

location of α7 subunit of nicotinic acetylcholine receptor

A

cortex

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10
Q

which enzyme carried out transcription?

A

RNA polymerase

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11
Q

alternative splicing occurs to…

A

mature mRNA

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12
Q

introns are removed from…

A

immature mRNA

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13
Q

properties of mature mRNA

A

5’ cap and polyA tail

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14
Q

Steps involved in cloning

A
  1. RNA isolation and fractionation
  2. mRNA to produce cDNA
  3. cDNA library construction
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15
Q

how is gene function determined?

A

electrophysiology

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16
Q

Expression cloning

A

plasmids used generate a library of clones, with each clone expressing one protein. This expression library is then screened for the property of interest and clones of interest are recovered for further analysis

17
Q

mammalian cloning techniques

A

Expression cloning
DNA probe
low stringency hybridisation

18
Q

ansiomycin

A

inhibits protein synthesis

19
Q

actinomysin D

A

inhibits RNA synthesis

20
Q

transcription factors can be either

A
21
Q

promoters are shared by

A

multiple genes

22
Q

the transcription factors TF2D and TBP bind to which promoter?

A

TATA

23
Q

what does TF2D recuit?

A

TF2B

24
Q

what does TB2B recruit?

A

TF2F and RNApol 2

25
Q

How do TF2E and TF2H activate transcription?

A

contain kinases which phosphorylate C tail of RNA pol

26
Q

repressors and enhancers

A

DNA sequences affecting transcription

Molecular Neuroscience (5 decks)

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