Fundamental approaches in molecular biology

Question 1

What happens in Affinity purification of RNA extraction?

Answer 1

After centrifugation and washing steps, nucleic acids bind to the silica membrane in the presence of high concentrations of chaotrophic salts.

Question 1

It prevents the emission of fluorophore through the quencher while the probe is intact

Answer 1

Fluorescence resonance energy transfer (FRET)

Question 2

Genome array method

Answer 2

One cDNA sample was labeled green (sample A) and another red (sample B)

Mixed and washed over a slide/chip with probes detecting gene expression

Each spot on the chip corresponds to a single gene

Spots glow with the color of fluorescent labels indicating gene expression levels