forensics lecture 6- bugs to bacteria Flashcards

1
Q

what is entomology?

A

-From the ancient Greek – entomon and -logia study of
-A branch of zoology.
-Study of insects – so those from the phylum arthopoda, with an exoskeleton, and no vertebrate.
-The largest group of any animal – more than 1million species have been described.
Insects live in nearly every conceivable environment and have done from the last 350 million years.
Entomology is crucial to our understanding of evolution, biodiversity, agriculture and human disease, but also forensics.

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2
Q

what is forensic entomology?

A

-the use of insects (and their larvae) and/or arthropods to aid in legal investigations
-primarily use to aid in unnatural death investigations

also used to
-Detect drugs and poisons
- Determine location of an incident
-Find the presence and time of the infliction of wounds

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3
Q

what are the 3 areas of application?

A

1- insects that inhabit human remains

2- insects that damage structures

3- infestation of foods

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4
Q

what is the post mortem interval?

A

the interval between time of death and post mortem exam

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5
Q

what are the methods used to determine PMI?

A

-Algor Mortis
-Livor Mortis
-Rigor Mortis
-Changes in fluid of the eye
-Stomach and bowel contents
-Decomposition changes
-Insect colonization
-Circumstantial evidence

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6
Q

how do insects provide evidence during post mortem interval?

A

-Season of death. -succession
-Changes in location after death – urban/rural
-Circumstances surrounding the death
-Body on the surface for a while before burial?
-Aquatic insects? Season and conditions under which the body came to be in water
-Toxicology

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7
Q

how are insects used as evidence in ecological concepts?

A

-Different species go through development in a predicted pattern.
-Developmental time is temperature dependent.
-Succession.

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8
Q

why are insects useful?

A

-metamorphosis
-feeding strategies
-sophisticated behaviour
-small size
-chitin
-wings
-life cycles

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9
Q

how are invertebrates used as forensic indicators?

A

1- invertebrates attracted to dead bodies eg detritivores
2- invertebrates that leave dead bodies
3- invertebrates that leave dead bodies
4- invertebrates as a cause of death

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10
Q

what is the entomology succession?

A

1- blowflies
2- flesh flies
3- burying/ histerid beetle
4- house flies
5- dermestid beetles

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11
Q

what insects colonise a corpse?

A

blue bottle fly

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12
Q

what is the life cycle of a blow fly?

A

-Adult female blowflies arrive within minutes to lay eggs on a body (up to 300 eggs) in wounds and natural openings
-Within 24 hours eggs hatch in to 1st instar larvae.
-Feed –moult to 2nd instar larvae
-Feed – moult to 3rd stage maggots
-Feed – moult to prepupae larvae move away from the body and no feeding
-Moult to pupae then final stage of moult to adult

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13
Q

why are microorganisms important?

A
  • advances in technology
  • awareness of dangers of pathogenic microbes
  • increase in individuals/ groups threatening to release pathogens maliciously and cause distres
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14
Q

what is the relationship between microorganisms and decomposition?

A

-We are host to a large number of microbes.
-Bacteria are important to the decay process.
-Environmental factors affect decomposition
Dry/frozen environmental slow down decomposition
Warm and moist environmental speed up decomposition.

-Time since death – can we use microorganisms for PMI? Not yet.

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15
Q

how are microorganisms used as identification tools?

A

-Soil microbes
-Saliva microbes
-Geographical origin
-Microbial infection transfer
-Food poisoning

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16
Q

how do you calculate the PMI?

A

1- use the average temperature to use the table information
2- find out how long it takes for the bug to colonise the body
3- find how long the first eggs take to hatch
4- calculate how long it takes for the 1st larvae to develop to 2nd larvae
5- then add how long it takes for the 2nd larvae to develop into 3rd larvae
6- see if the larvae are mid range then divide the hours by 2

tips= turn it into days if needed and remember there is a range due to the mix of last larvae

17
Q

what is the fresh stage of decomposition?

A

body starts to cool =
Blowfly eggs and 1st instar larvae
Fleshfly 1st nstar larvae Burying beetle adults

18
Q

what is the bloat stage of decomposition?

A

discolouration and body swelling=
Blowfly eggs + 1st , 2nd , 3rd instar larvae
Fleshfly 1st , 2nd , 3rd instar larvae
Burying beetle adults and larvae, Histerid beetle adults and larvae

19
Q

what is the putrefaction stage of decomposition?

A

loss of skin and soft tissue=
No blowfly eggs once advanced putrefaction, Blowfly 2nd , 3rd instar larvae, Fleshfly 2nd , 3rd instar larvae
Blowfly & fleshfly larvae leaving corpse for pupation site
Histerid beetle adults and larvae, Eristalid fly larvae (liquefied regions),Phorid fly larvae (later stages of putrefaction)
Piophilid fly larvae (later stages of putrefaction)

20
Q

what is the putrid dry remains stage of decomposition?

A

slow progress of decay =
No blowfly eggs once advanced putrefaction, Blowfl y 2nd , 3rd instar larvae, Fleshfly 2nd, 3rd instar larvae
Blowfly & fleshfly larvae leaving corpse for pupation site
Histerid beetle adults and larvae, Eristalid fly larvae (liquefied regions)
Phorid fly larvae (later stages of putrefaction)
Piophilid fly larvae (later stages of putrefaction)