for pa lab Flashcards
Study of the interaction of LIGHT/EMR with matter
Spectroscopy
Involves measurement of the amount of EMR that is absorbed, emitted or scattered by a sample to perform an assay
Spectroscopy
Spectroscopy is quantitative in nature
TRUE
a branch of electromagnetic spectroscopy concerned with the quantitative measurement of the reflection or transmission properties of a material as a function of wavelength.
Spectrophotometry
Pharmacopoeial methods rely heavily on simple analysis by ______ to determine active ingredients in formulations.
UV/visible spectrophotometry
Instrumentation of Spectroscopy
Light Source
Wavelength Isolator
Sample Cell/Cuvette
Detector
Signal Processing and Readout Unit
Radiation Sources/Light Sources
Deuterium Lamp
Hydrogen Lamp
Tungsten Filament Lamp
Xenon Arc Lamp
It isolates a limited region of the
spectrum of measurements
Wavelength Isolator
It converts radiant energy to a measurable electrical signal
Detector
This device enable the selection of relatively narrow bond of wavelengths from a broad brand of radiation.
Wavelength Selector
Prevent passage of radiation at all wavelengths except in a fixed wavelength region.
Filters
Methods of Quality System for the Analytical Laboratory
Uv and Visible Spectrophotometry
NIR Spectrometry
MID IR Spectrometry
Fluorescence Soectrometry
It is one of the most widely used techniques in analytical chemistry, capable of producing accurate and precise results. For these reasons, procedures using this technique are found in analytical, clinical, and research laboratories, and find extensive use in quality assurance.
UV-Vis Spectrophotometry
It is used routinely to monitor in vitro release of active ingredients from formulations.
UV Spectrophotometry
NIR radiation has good penetration properties and thus minimal sample preparation is required and thick sample layers can be used to compensate for the weakness of NIR absorption
NIR Spectrometry