Food Tests Flashcards
Test for starch
Add 3-4 drops of iodine solution (be specific if testing qualitatively) Colour change from reddish brown to blue black is positive. Faster colour change/deeper shade is more starch
Test for reducing sugars
Make a solution of the substance being tested Adan equal volume of Benedictus solution. Heat in a boiling water bath colour change from blue to cloudy green to yellow to red is positive. Faster colour change and deeper shades indicates higher amounts
Test for Protein
Add 5cm3 of sodium hydroxide and 5cm3 of 1%copper sulphate solution (biuret solution) to the food. Colour change of blue to purple indicates protein, the deeper the shade of purple the more protein.
Test for fats/oils
Crush food in ethanol and filter
Pour this solution into a test tube containing a few cm3 of water, a white milky emulsion indicates the presence of fats/oils
Test for Vitamin C
Add liquid drop by drop into 2cm3 of 0.1% DCPIP. Colour change of blue to colourless indicates presence of Vitamin C. The fewer the drops the more vitamin c present