Food Tests Flashcards

1
Q

testing for fats

A

the emulsion test

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1
Q

The emulsion test (procedure)

A

add ethanol to the sample and shake, transfer the solution and add water if a lipid is present then a milky white layer will form

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2
Q

testing for protein

A

the biuret test

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3
Q

Biuret test (procedure)

A

add the sample to biuret solution, shake and then leave for 5 minutes, if a protein is present the solution will change from a blue to lilac

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4
Q

testing for reducing sugars

A

benedicts test

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5
Q

benedicts test (procedure)

A

add benedics solution to the sample and place in a water bath for around 5 minutes, if there is a reducing sugar then the solution will turn from blue to yellow-brick red dependent on the concentration of the sugar

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6
Q

testing for non reducing sugars

A

benedicts test, hydrolyse the solution and repeat the benedicts test

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7
Q

testing for reducing sugars (procedure)

A

use the benedicts test to ensure that the sugar is non-reducing, the colour should not change to begin with, add hydrochloric acid to hydrolyse the solution and put into a water bath, after 5 minutes neuralise with sodium bicarbonate and then repeat the benedicts test, if a reducing sugar is present then the colour should change from blue to red/orange

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8
Q

testing for starch

A

potassium iodide test

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9
Q

potassium iodide test (procedure)

A

add potassium iodide to the sample and if a starch is present then the solution will change from orange to blue-black

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