Food Tests Flashcards
testing for fats
the emulsion test
The emulsion test (procedure)
add ethanol to the sample and shake, transfer the solution and add water if a lipid is present then a milky white layer will form
testing for protein
the biuret test
Biuret test (procedure)
add the sample to biuret solution, shake and then leave for 5 minutes, if a protein is present the solution will change from a blue to lilac
testing for reducing sugars
benedicts test
benedicts test (procedure)
add benedics solution to the sample and place in a water bath for around 5 minutes, if there is a reducing sugar then the solution will turn from blue to yellow-brick red dependent on the concentration of the sugar
testing for non reducing sugars
benedicts test, hydrolyse the solution and repeat the benedicts test
testing for reducing sugars (procedure)
use the benedicts test to ensure that the sugar is non-reducing, the colour should not change to begin with, add hydrochloric acid to hydrolyse the solution and put into a water bath, after 5 minutes neuralise with sodium bicarbonate and then repeat the benedicts test, if a reducing sugar is present then the colour should change from blue to red/orange
testing for starch
potassium iodide test
potassium iodide test (procedure)
add potassium iodide to the sample and if a starch is present then the solution will change from orange to blue-black