FMBG 5 -class notes transcription/translation Flashcards
Nascent RNA ____ (remains hybridized, does not remain hybridized) to the template DNA
does not remain
RNA polymerase ____ (needs, does not need) a primer
does not need
RNA transcription is ____ (less, more) accurate than DNA replication
much less accurate
The template strand is read ___ to ____, synthesizing RNA ___ to ____
the non-template strand has the ___ sequence as the RNA
3’ to 5’
5’ to 3’
same (AKA the sense strand)
RNA polymerase I makes ____, and is about ___% of RNA synthesis activity
5.8S, 18S, and 28S rRNAs
70%
RNA polymerase III synthesizes ___ and ___, as well as some ___ and ____
tRNA, 5S RNA
some snRNA (splicing, poly(A))
scRNA (signal recognition particle)
RNA polymerase II synthesizes ___, ___ and some ____
mRNA, snoRNA, and some snRNA
messenger RNA is made by RNA polymerase ____
II
The transcription factor complex includes the ___, which binds the TATA box
TBP (tata binding protein)
The CTD (c-terminal domain) of the RNA polymerase II gets phosphorylated by _____
helicase
the ____ of RNA contains the whole genome, and is the substrate for splicing
primary transcript
in bacteria, the half life of RNA is ___
couple minutes
in eukaryotes, the half life of RNA is ____
variable - minutes to tens of hours
The ___ aids in mRNA nuclear export and protects from 5’->3’ exonuclease degradation in the cytoplasm.
It also targets transcripts to the ribosome for translation
5’ cap
the ____ participates in the termination of transcription, aids in mRNA nuclear export, and protects from 3’ to 5’ exonuclease degradation in the cytoplasm.
It also targets the transcripts to the ribosome for translation
poly A tail
