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FOOD TECHNOLOGY > FMB - Lab Lecture 1 > Flashcards

FMB - Lab Lecture 1 Flashcards

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1
Q

Used for handling, isolating, transferring, and cultivating microbes.

A

Inoculating Loop

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2
Q

Used for stabbing bacteria vertically into deep tube of media to determine the shape of the hole digested by the bacteria.

A

Inoculating Needle

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3
Q

Used for spreading the bacteria in the plate.

A

L-shape glass rod

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4
Q

Can be used as spreader and can also be used during sampling instead of inoculating loops.

A

Cotton Swab

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5
Q

Allow the users to extract or deliver small amount of liquid of microbial dilutions.

  • micro liter
  • “exact quantity”
A

Mechanical pipettes

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6
Q

Used in heating substances and in incineration the specialized tool to avoid contaminants.

A

Alcohol Lamp

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7
Q

Used for the cultivation of microorganism on solid media.

A

Petri plates

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8
Q

Can be used for culturing, maintaining and storing cultures, specifically in broth.

A

Test Tubes

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9
Q

Important in microbial dilutions for microbial count.

A

Test Tubes

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10
Q

Hold the microbial cultures and store liquids.

A

Erlenmeyer Flask

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11
Q

Can also be used for the preparation of culture media.

A

Erlenmeyer Flask

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12
Q

Hold and work in mixing culture media and other liquids.

A

Beaker

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13
Q

Not recommended in culturing bacteria due to its open space or wide mouth.

A

Beaker

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14
Q

Latin word of Microscope

A

Microscopium

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15
Q

“to look at”

A

skopein

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16
Q

A laboratory instrument used to examine objects that are to small to be seen by the naked eye.

A

Microscope

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17
Q

Contains more than one magnifying lens.

A

Compound Light Microscope

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18
Q

The image from the _________ is magnified again by the __________ .

A
  1. Objective Lens
  2. Ocular Lens
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19
Q

Maximum magnifying power of about ________.

A

1,000 x

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20
Q

Specimen remain in focus.

A

Parfocal

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21
Q

Remagnifies the image formed by the objective lens.

A

Ocular lens

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22
Q

Transmit the image from the objective lens to the ocular lens.

A

Body tube

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23
Q

Primary lenses that magnify the specimen.

A

Objective lenses

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24
Q

Holds the microscope slide in position.

A

Stage

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25
Q

Focuses light through the specimen.

A

Condenser

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26
Q

Controls the amount of light entering the condenser.

A

Diaphragm

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27
Q

Light source.

A

Illuminator

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28
Q

A device that uses steam to sterilize equipment and other objects. This means that all bacteria, viruses, fungi, and spores are inactivated.

A

Autoclave

29
Q

It is a pressurized device that enables the heating of aqueous solutions up to temperatures above the boiling point of water.

A

Autoclave

30
Q

It works by allowing steam to enter and maintaining
pressure of ________ . This causes the steam to reach _________ for ________.

A
  1. 15 psi
  2. 121 degree C
  3. 15 Minutes
31
Q

When the holding period is over, the heater is turned off and the autoclave allowed to cool till the pressure inside is equal to the atmospheric pressure.

A

Autoclave

32
Q

Provide a high degree of localized control for critical processes.

A

Laminar flow hood

33
Q

The work area is continuously bathed with positive pressure HEPA- filtered air to protect the product from contamination.

A

Laminar flow hood

34
Q

Designed for the handling of materials in a sterile working environment.

A

Laminar flow hood

35
Q

A device used to grow and maintain microbiological cultures or cell cultures.

A

Incubator

36
Q

Maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.

A

Incubator

37
Q

Highly sensitive lab instruments designed to accurately measure mass.

A

Analytical Balance

38
Q
  • have finer readability,
  • more sensitive to changes, and
  • can detect smaller variations in mass
A

Analytical Balance

39
Q

Can safely be used in microbiology
laboratory for preparation of media.

A

Microwave

40
Q

It may be used to preserve certain chemical reagents or media.

A

Refrigerator

41
Q

It may be used to grow certain types of bacteria.

A

Refrigerator

42
Q

This can also be used to preserve cultures of bacteria, yeast, and other microbes.

A

Refrigerator

43
Q

Cooked cut potato - earliest solid medium

A

Robert Koch

44
Q
  • not satisfactory due to low melting point
  • liquefy at 24 degree C
A

Gelatin

45
Q

Agar

A

Frau Hesse

46
Q
  • Most used to prepare solid media
  • Polysaccharide extract obtained from seaweed.
  • No nutritive value
  • Not affected by the growth of the bacteria.
A

Agar

47
Q

Melts at ___ to ___ & sets at ___ to ____ .

A

Melts at 98 to 100 degree C & sets at 42 to 45 degree C.

48
Q

_____ agar is employed in solid medium

A

2%

49
Q

AGAR: AN IDEAL SOLIDIFYING AGENT

A

(a) Bacteriologically inert.
(b) No influence on bacterial growth.
(c) It remains solid at 37°C.
(d) It is transparent.

50
Q

Exact composition is not known.

A

Empirical or Natural

51
Q

Have known quantities of all ingredients.

A

Synthetic or Defined

52
Q

Empirical or Natural examples.

A

milk, urine, diluted blood, vegetable juices, meat
extracts, infusions and peptone.

53
Q

Synthetic or Defined

A

Glucose or glycerol are often used as carbon sources, and ammonium salts or nitrates as inorganic nitrogen sources.

54
Q

Liquid (broth)

A
  • used for profuse growth
  • Mixed organisms cannot be separated.
55
Q

Solid (slant, plate)

A
  • Bacteria may be identified by studying the colony character.
  • Mixed bacteria can be separated.
  • Solid media is used for the isolation of bacteria as pure culture.
56
Q

Semi-solid (soft agar)

A
  • Contains 0.5% agar
  • Usually for motility media
  • Isolation of bacteriophage
57
Q

May be used for growth (culture) of bacteria that do
not need enrichment of the media.

A

General or Non-selective

58
Q

General or Non-selective example.

A

Enterobacteriaceae and Staphylococcus spp.

59
Q

Macroscopically visible collection of millions of bacteria originating from a single bacterial cell.

A

Colony

60
Q

Favors the growth of a particular bacterium by inhibiting the growth of undesired bacteria and allowing growth of desirable bacteria.

A

Selective media

61
Q

uses the biochemical characteristics of a
microorganism growing in the presence of
specific nutrients or indicators added to the
medium to visibly indicate the defining
characteristics of a microorganism.

A

Differential

62
Q

Lactose Fermenting Colonies

A

Pink

63
Q

Non-lactose Fermenting Colonies

A

Colorless

64
Q

MacConkey’s Agar

A

Gram negative bacteria

65
Q

Macrospically visible collection of millions of bacteria originating from a single bacterial cell. 25-200 CFU’s

A

Colony

66
Q

Mechanical Parts of Microscope:

A
  • Base
  • Pillar
  • Arm
  • Stage
  • Inclination Joint
  • Stage clips
  • Revolving nose piece
  • Body Tube
  • Draw Tube
  • Fine ajdustment knob
  • Course adjustment knob
67
Q

Optical Parts of Microscope:

A
  • Objective Lens
  • Ocular Lens
68
Q

Illuminating Parts of Microscope:

A
  • Diaphragm
  • Mirror

FOOD TECHNOLOGY (9 decks)

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