Flow Review

Question 1

Light scatter placed where?

Flourescence where?

Forward scatter tells us?
Side scatter?

Answer 1

Behind and 90 degree

90 degree

Forward: Size

Side: Granulatity

Question 2

Lack of CD3 associated with?

Lack of CD19?

Answer 2

DiGeorge

BXLA

Question 3

T/F % of cells in S phase is indicative of node negative breast cancer?

T/F Diploidy is poor prognositic indicator of neuroblastoma?

Answer 3

True

True

Question 4

What dye binds to DNA and use?

Answer 4

Propidium Iodide that stains double stranded nucleic acid (need to break down double stranded RNA; RNAse) in stoichiometric ratio: Used to see if cell is mitotic or not

Get G0-G1 peak and G2-M peak with S in between

Can also look at Aneuploidy

Question 6

What is a flow cytometry cross match?

Ab to HLA class 1 react with?
Ab to HLA class 2 react with?

Answer 6

Pretransplant
Recipient’s serum with donor cells; add anti-human (g and flourescent molecule

HLA Class 1: T and B

HLA Class 2: B cells only

Question 7

What marker can be used to look for stem cells?

ISHAGE Protocol uses what 2 antibodies?

Answer 7

CD34 in patients treated with colony stimulating factor

anti-CD34 (stem cells) and anti-CD45 (gating)

Question 8

What is the flow assay for Neutrophil function?

What is NOT seen in patients with chronic granulomatous disease?

What do you gate on?

Answer 8

PMA activates neutrophils to reduce Rhodamine (TRITC)

Oxidative burst following PMA activation

Neutrophils

Question 9

PNH see decrease in what CD’s?

More modern CD to examine and test assay?

Answer 9

CD55 and CD59!

See decreased FLAER, CD157; high-sensitivit for PNH

Question 10

Absolute count formula?

Answer 10

Cell counted/ (original specifimen volume * % of beads counted)