AML-Rosenblum Flashcards
Blast equivalents?
Myeloblast Markers
Promonocyte (ALWAYS); Promyelocytes in APL
Myeloblast: HLA-DR,CD33, CD13, MPO, CD34
Promyelocyte markers?
Monoblast/Promonocyte markers?
Erythroblast markers?
Megakaryoblast markers?
Promyelocyte: MPO, CD33, CD13
Monoblast/Promonocyte: NSE+, HLA-DR, CD33, CD13, CD36, CD64
Erythroblast: PAS, Glycoophorin A, Hgb A, CD71
Megakaryoblast: PAS, CD31, CD41, CD61, CD42a
Favorable AML cytogentics (3)?
Intermediate (4)?
Unfavorable (4)?
t(8;21), t(15;17), inv (16)
Normal, +8, +21, 11q23
-5. -7, 3q, complex
Cytonormal AML mutations (bad):
FLT3-ITD ch13 prognosis?
MLL-PTD?
BAALC over expression ch21;23?
ERG overexpression ch21?
FLT3-ITD ch13: Poor prognosiss
MLL-PTD: Short relapse
BAALC: worse CR and shorter survival
ERG: Shorter OS and higher CIR
Cytonormal AML mutations (favorable):
NPM1 mutations ch2;5?
CEBPA mutations ch19?
NPM1 mutation without FLT3-ITD better CR, OS
CEBPA: better CRD and OS than those without
FAB M0?
Morphology, Auer Rods?
MPO and NSE +/-?
Flow markers?
Non-specific without differentiation, NO AUER RODS
<3%+ for MPO and NSE
CD34+, CD38+, HLA-DR+
CD117+, CD13+, CD33+, MPO +
FAB M1?
Morphology?
MPO +/-?
Flow markers?
Show myeloid diff but lack maturation: Cytoiplasmic azurophilic granules and possible Auer Rods
Maturing granulocytes <10%
MPO or SBB >3%
CD34+, HLA-DR+, CD13+, CD33+, CD117+,
May be +: CD7, CD2, CD4, CD19, CD56
Negative: CD15 and CD65 (myeloid maturation), , monocytic CD14 and CD64
FAB M2?
Morphology (and percent maturation)?
Markers?
Morphologic and cytochemical evidence of maturation (>10% maturing cells)
Blasts with azurophilic granules +/- Auer Rods; <20% Monocytes and pecursers
Spectrum: CD34,CD 117, HLA-DR
Multiple myeloid lineage markers (+): CD13, CD33, CD11c, CD65, CD15
10-30% (+): CD7, CD56, CD2, CD19, CD4
Negative for monocytic: CD14, CD36, CD64
FAB M4?
Morphology?
Markers?
Cytogenetic finding?
Acute myelomonocytic leukema:
>20% blasts (including promonocytess, >20% neutorphils and precursors, >20% monocytes and precursors
Subset (+): CD34, CD117, HLA-DR
Subset (+) myeloid markers: CD13, CD33, CD15, CD65
Subset monocytic (+): CD14, CD64, CD36,CD4, CD11b, CD11c
No specific abnormalities, +8 common
FAB M5?
Morphology?
IHC markers?
Flow markers required for diagnosis?
Acute monoblastsic and monocytic! ; poor prognosis
>80% monocytes with monoblastic blast morphology, possible vaculoes and granules
Monocytic: mature chromatin and folded and indented
NSE +, CD68+, CD163+
At least 2 by flow: CD14, CD4, CD11b, CD11c, CD64, CD36
FAB M6?
Morphology?
Markers?
Pure Erythroid Leukemia, aggresssive
Pronormoblasts: round, fine chromatin, nucleolous, DEEPLY BASOPHILIC CYTOPLASM, vacuoles
PAS +, Hb A, glycophphorin (CD235a), spectrin, e-Cadherin, CD71
Negativce: CD34, CD117. HLA-DR
FAB M7?
Morphology?
Markers?
Occurs with?
Megakaryoblastic; poor prognosis
Medium to large with dense chromatin with BLEBS
Flow (1+): CD41, CD61, CD42
Neg: MPO, CD45, HLA-DR, TdT, and lymphoid
Germ cell tumors!
AML RUNX1-RUNX1T1 is what translocation?
Morphology?
Markerss?
Fusion or not?
Prognosis?
t(8;21)(q22;q22.1)
Larg blasts, maturing, Auer rods
CD13+, CD33+, CD117+, CD34+, CD19+ and CD66+
Fusion: RunX1/RUNX1T1, KIT
Good; high CR
What are the two Ch16 AML’s?
Morphology
Markers?
Outcome?
INV(16)(P13.1;q22); T(16;16)(P13.1;q22); CBFB-MYH11
Abnormal Eosinophils in BM; AMML, EO/BAS Cells
CD13, CD33, CD14, CD4, CD64
Favorable
Promyelocytic leukemia transolcation?
Morphology?
Markers?
Outcome?
t(15;17) PML/RARA; Faggot Cells
Hypergranular promyelocytes (low WBC); Microglandular: folded nuclei (high WBC)
My antigen +, HLA/DR -, CD34-, MPO+, CD33+, CD13+, CD45 dim, CD64 dim, HIGH SIDE SCATTER
Favorable; Coagulopathy possible
