Flow Markers

Question 1

AML monocytes cell surface markers

Answer 1

NSE
Cd11
Cd14
Cd64
Lysozyme
Cd4
Cd11b
Cd36

Question 2

AML megakaryocytes cell surface markers

Answer 2

Cd41
Cd61
Cd42

Question 3

AML erythroid cell surface markers

Answer 3

Cd235a

Question 4

Precursor B Lymphoblastic Leukemia/Lymphoma

Answer 4

DR, CD19, CD20-/+, CD34, CD10+/-, sIg-, TdT+
May co-express myeloid Ag in t(9;22)
Infants w/CD10-,CD15+ suspect 11q23

Question 5

Burkitt

Answer 5

DR, CD19, CD20, CD22, CD10+/-, CD34-, TdT-, sIg+ (bright)

Question 6

CLL/SLL

Answer 6

DR, CD19, CD20 (dim), CD5, CD23, CD45, CD22-, CD10-, FMC7-, CD11c+/-, CD25+/-, sIg (dim to negative)
If bright sIg+, CD20+ suspect Trisomy 12

Question 7

B Prolymphocytic Leukemia

Answer 7

DR, CD19, CD20 (bright), CD22, CD5-, CD23-, CD10-, sIg+ (bright)

Question 8

Mantle Cell Lymphoma

Answer 8

DR, CD19, CD20, CD5, CD22, sIg+, CD43, CD23-, CD10-

Question 9

Follicular Lymphoma

Answer 9

DR, CD19, CD20, CD10, CD22, CD5-, CD23+/-, CD11c-, CD43-, sIg+ (bright)
20% of cases are CD10-

Question 10

Marginal Zone Lymphoma

Answer 10

DR, CD19, CD20, CD22, CD11c, sIg+, CD43+/-, CD5-, CD23-, CD10-, CD25-, CD103-
sIgD rare

Question 11

Splenic Marginal Zone Lymphoma

Answer 11

DR, CD19, CD20, CD22, CD11c, sIg+, CD43-, CD5-, CD23-, CD10-, CD25-
sIgD+

Question 12

Lymphoplasmacytic Lymphoma

Answer 12

DR, CD19, CD20, CD22, sIg+, CD43+/-, CD5-, CD10-, CD23-

Question 13

Hairy Cell Leukemia

Answer 13

DR, CD19, CD20 (bright), CD22, CD25, sIg+, CD11c (bright), CD103, CD5-, CD10-, CD23-

Question 14

Plasma Cell Myeloma

Answer 14

CD45, CD38 (bright), cIg+, sIg+/-, DR-, CD19-, CD20-, CD22-

CD56 often

Question 15

Precursor T Lymphoblastic Leukemia/Lymphoma

Answer 15

cCD3, CD4, CD8, TdT, CD10-/+

May co-express multiple myeloid Ag

Question 16

T Prolymphocytic Leukemia

Answer 16

CD2, CD3, CD5, CD7, CD4+/-, CD8-/+

Dual CD4+/CD8+ or CD4-/CD8+ rare

Question 17

MF/SS

Answer 17

CD2, CD3, CD5, CD4, CD7-/+, CD8-, CD25-

May have no aberrant phenotype

Question 18

Adult T-Cell Leukemia/Lymphoma

Answer 18

CD2, CD3, CD5, CD4, DR, CD25 (bright), CD7-, CD8-

Question 19

T-Cell LGL Leukemia

Answer 19

CD2, CD3, CD8, CD11b, CD57, CD56-/+, CD4-, CD25-

CD56+ more aggressive

Question 20

NK-Cell LGL Leukemia

Answer 20

CD2, CD16, CD56, CD8-/+, sCD3-, cCD3+, CD4-, CD57-, CD25-

Question 21

Anaplastic Large Cell Lymphoma

Answer 21

CD3+/-, CD4+/-, CD5+/-, CD7-/+, CD8-, CD25+, CD30+ (bright)

Question 22

Peripheral T-Cell Lymphoma, NOS

Answer 22

CD3+, CD4+/-, CD8-/+, CD5-, CD7-/+

Question 23

CD2

Answer 23

Monocytes (dim)
NK Cells (or partial CD2)

Question 24

CD4

Answer 24

Monocytes (dim)

Question 25

CD7

Answer 25

NK Cells

Question 26

CD9

Answer 26

Basophils

Mast Cells

Question 27

CD8

Answer 27

NK Cells (dim)(+/-)

Question 28

CD10

Answer 28

Monocytes (dim)

Neutrophils

Question 29

CD11b

Answer 29

Monocytes (bright)
NK Cells
Basophils
Neutrophils

Question 30

CD11c

Answer 30

Monocytes (bright)
NK Cells
Basophils
Neutrophils

Question 31

CD13

Answer 31

Monocytes
Basophils
Neutrophils

Question 32

CD14

Answer 32

Monocytes

Question 33

CD15

Answer 33

Monocytes (dim)
Basophils
Neutrophils

Question 34

CD16

Answer 34

Monocytes (dim)
NK Cells
Basophils
Neutrophils

Question 35

CD22

Answer 35

Basophils

? Monocytes ?

Question 36

CD33

Answer 36

Monocytes
Basophils (dim)
Mast Cells

Question 37

CD25

Answer 37

Basophils

Question 38

CD36

Answer 38

Monocytes
NK Cells
Basophils
nRBCs

Question 39

CD38

Answer 39

Monocytes
NK Cells
Basophils

Question 40

CD45

Answer 40

Monocytes (bright)
Mast Cells
Basophils (dim)
nRBCs (dim to negative)

Question 41

CD56

Answer 41

NK Cells

Question 42

CD57

Answer 42

NK Cells

Question 43

FMC-7

Answer 43

Monocytes (dim)

Question 44

HLA-DR

Answer 44

Monocytes

NOT seen on Basophils

Question 45

CD68

Answer 45

Mast Cells

Question 46

CD117

Answer 46

Mast Cells

Question 47

B & T Cells

Answer 47

Negative for:
CD14
CD15
CD16
CD34