Fixation (II: Compound Fixatives) Flashcards
Fixation time for 10% formol-saline
12-24 hours
For preservation of post-mortem surgical research specimens
10% buffered formalin
Fixation time for 10% buffered formalin
24 hours or more
For biopsies of the skin
Heidenhain’s Susa Solution
Fixation time for Haidenhain’s Susa Solution
3-12 hours
For routine post-mortem materials
Formol sublimate
Fixation time for formol sublimate
3-24 hours
Does not preserve thick slices of tissue; not to be used for than 1 cm thick sections
Formol sublimate
For post-mortem materials
Formol saline sublimate
Zenker’s solution
Recommended for tissues to be stained by one of the trichrome technique
Zenker’s solution
For pituitary tissues and bone marrow
Zenker’s formol (Helly’s fluid)
Prolonged fixation produces brown scum
Zenker’a formol (Hellyy’s fluid)
For embryos and glycogen
Bouin’s solution
Should never be used for preserving kidneys due to extreme distortion
Bouin’s solution
Recommended for nuclear structures (chromosomes)
Flemming’s fluid