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Histopathology (RVSP) > Clearing > Flashcards

Clearing Flashcards

1
Q

Clearing agents must be miscible with _________ & __________

A

Alcohol

Paraffin wax

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2
Q

Process of removing alcohol from section of tissue by immersing them in ante-medium

A

Dealcoholization/Clearing

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3
Q

Best routine clearing agent for urgent biopsies

A

Benzene

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4
Q

Toxic to liver after prolonged inhalation

A

Chloroform

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5
Q

Suitable for large tissue specimens, tough tissues, nervous tissues, lymph nodes and embryos

A

Chloroform

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6
Q

Becomes milky if incompletely dehydrated tissue is immersed

A

Xylene

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7
Q

Best clearing agent

A

Cedar Oil

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8
Q

Recommended for CNS, skin and smooth muscle, and cytological work

A

Cedar oil

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9
Q

Recommended for skin and smooth muscle

A

Clove oil

Oil of bergamot

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10
Q

Clove oil is mixed with ___________ to prevent tissue brittling

A

Oil of Bergamot

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11
Q

Does not extract aniline dyes; can be used with celloidin preparations

A

Oil of bergamot

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12
Q

Recommended for skin

A

Oil of Origanum (Spanish hop oil/Thyme oil)

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13
Q

Recommended for delicate tissues

A

Oil of Wintergreen

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14
Q

Mainly for celloidin sections but must be soaked out by chloroform/xylene before mounting

A

Oil of Wintergreen

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15
Q

Oxidizes easily on standing

A

Oil of Wintergreen

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16
Q

Recommended for smooth muscles

A

Carbon disulfide

17
Q

Recommended for friable tissues

A

Carbol-xylene

18
Q

Good for moist and humid climates

A

Carbol-xylene

19
Q

Made by adding anhydrous crystals of phenol to xylene until no more dissolves

A

Carbol-xylene

20
Q

Recommended for cellular organs, friable tissues, skin, athermatous arteries ams hard collagenous specimens

A

Tetrahydrofuran

21
Q

Its fumes may cause conjunctival irritation after about 6 months

A

Tetrahydrofuran

22
Q

Superior to ordinary clearing agents due to its ability to perform 2 processes at the same time

A

Tetrahydrofuran

23
Q

Recommended for delicate material especially the eyes

A

Terpineol

24
Q

Recommended for smooth muscles; good for paraffin and celloidin work

A

Phenol

25
Q

Dehydrating agents that cause the least hardening of tissues

A

Cedar oil
Gasoline
Petrolium ether

Histopathology (RVSP) (10 decks)

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