Clearing Flashcards
Clearing agents must be miscible with _________ & __________
Alcohol
Paraffin wax
Process of removing alcohol from section of tissue by immersing them in ante-medium
Dealcoholization/Clearing
Best routine clearing agent for urgent biopsies
Benzene
Toxic to liver after prolonged inhalation
Chloroform
Suitable for large tissue specimens, tough tissues, nervous tissues, lymph nodes and embryos
Chloroform
Becomes milky if incompletely dehydrated tissue is immersed
Xylene
Best clearing agent
Cedar Oil
Recommended for CNS, skin and smooth muscle, and cytological work
Cedar oil
Recommended for skin and smooth muscle
Clove oil
Oil of bergamot
Clove oil is mixed with ___________ to prevent tissue brittling
Oil of Bergamot
Does not extract aniline dyes; can be used with celloidin preparations
Oil of bergamot
Recommended for skin
Oil of Origanum (Spanish hop oil/Thyme oil)
Recommended for delicate tissues
Oil of Wintergreen
Mainly for celloidin sections but must be soaked out by chloroform/xylene before mounting
Oil of Wintergreen
Oxidizes easily on standing
Oil of Wintergreen
Recommended for smooth muscles
Carbon disulfide
Recommended for friable tissues
Carbol-xylene
Good for moist and humid climates
Carbol-xylene
Made by adding anhydrous crystals of phenol to xylene until no more dissolves
Carbol-xylene
Recommended for cellular organs, friable tissues, skin, athermatous arteries ams hard collagenous specimens
Tetrahydrofuran
Its fumes may cause conjunctival irritation after about 6 months
Tetrahydrofuran
Superior to ordinary clearing agents due to its ability to perform 2 processes at the same time
Tetrahydrofuran
Recommended for delicate material especially the eyes
Terpineol
Recommended for smooth muscles; good for paraffin and celloidin work
Phenol
Dehydrating agents that cause the least hardening of tissues
Cedar oil
Gasoline
Petrolium ether
