Fixation/Histo Lecture

Question 1

How does fixation allow for contrast between tissues?

Answer 1

By changing the refractive index of the tissue

Question 2

T or F:

Fixation stops autolysis by bacT and enzymatic agents

Answer 2

True

Question 3

If a tissue is not fixated properly, what could be a detrimental result?

Answer 3

Lack of definition because the stain won’t take well

Question 4

Best fixation for autolyzed tissue (like eyes, nervous, etc)

Answer 4

Injectable formalin or Bouin’s/Davidson’s

Question 5

Ideal thickness of tissue in cassette during trimming ?

Answer 5

3 mm *