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MBD > FINALS - Molecular Clinical Laboratory > Flashcards

FINALS - Molecular Clinical Laboratory Flashcards

1
Q

begins with EVALUATION, SELECTION, and IMPLEMENTATION of appropriate DIAGNOSTIC tests

A

1st step of process of diagnostic analysis

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2
Q

incorporation GUIDANCE for healthcare providers regarding the use of testing for appropriate patients

A

2nd step of process of diagnostic analysis

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3
Q

ensure TIMELY sample collection, transport, and processing, and timely reporting of results

A

3rd/final step of process of diagnostic analysis

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4
Q

aim at quality, format, and amount of specimen/sample, which should be DOCUMENTED in DETAIL

A

pre analytical standards

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5
Q

consider the proof of these aspects, concerning DNA/RNA extraction, quantification, and purity determination via FLUOROMETRY/SPECTROPHOTOMETRY

A

analytical standards

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6
Q

mainly focuses on the documentation and BIOINFORMATICS PROCESS

A

post analytical analysis

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7
Q

the number one fixing reagent in histopath lab is?

A

formalin

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8
Q

this may reduce DNA and particularly RNA recovery

A

decalcification

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9
Q

this means you are REMOVING the CALCIUM from a tissue

A

decalcification

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10
Q

measures total nucleic acids as well as impurities that markedly overestimate amplifiable DNA or RNA content

A

spectrophotometric methods

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11
Q

true or false:

spectrophotometer reads all nucleic acid contents including impurities so it give OVERPOPULATED results

A

true

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12
Q

what is the best platform to use for cerebrospinal fluid testing?

A

multiplex PCR platforms

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13
Q

recommended pH for samples

A

more than 6 or less than 9

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14
Q

temperature for storing DNA and RNA samples

A

-20 degrees C or lower

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15
Q

used almost exclusively for quality control in sequencing projects because it can be used to INVESTIGATE FRAGMENT SIZE distribution and final library quality assessment

A

capillary gel electrophoresis

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16
Q

used commonly to determine the INPUT DNA/RNA amount and to CHECK SAMPLE QUALITY at appropriate steps of the sequencing or PCR workflow

A

fluorometer

17
Q

the TIMELINESS of reports is CRITICAL to patient care and depends on patient pathways in practice in individual hospital

A

TAT

18
Q

time/period on where the molecular test releases the result

A

TAT

19
Q

also known as PROFICIENCY TESTING, are inevitable for monitoring performance of the lab

A

EQA

20
Q

this is necessary to ensure HIGH ASSAY REPRODUCIBILITY and performance and enable detection and correction of errors in daily practice

A

IQA

MBD (5 decks)

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