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MBD > FINALS - ELISA > Flashcards

FINALS - ELISA Flashcards

1
Q

any IMMUNOASSAY that uses an ENZYME as label

A

enzyme immunoassay

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2
Q

reagent Ag or Ab bound to support medium

A

solid phase EIA

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3
Q

true or false:

in indirect ELISA, the darker the color the lower the presence of the target antibody

A

false (the HIGHER)

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4
Q

application of indirect ELISA: used to detect Abs to viruses

A

immunology and serology

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5
Q

application of indirect ELISA: used to analyze proteins

A

molecular biology

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6
Q

what is the primary antibody in indirect ELISA?

A

patient antibody

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7
Q

if the antibody present is NOT specific to the antigen, it is called ____

A

unbound antibody

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8
Q

an antibody specific for the antibody that is being targeted by test

A

enzyme-labeled anti-immunoglobulin

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9
Q

the targeted antibody in the test is present when there is a color change, hence ____

A

positive

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10
Q

when there is NO color change, the test is ____

A

negative

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11
Q

content of yellow tube

A

student samples (serum)

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12
Q

content of green tube

A

purified antigen

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13
Q

content of violet tube

A

positive control

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14
Q

content of blue tube

A

negative control

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15
Q

content of orange tube

A

secondary antibody (enzyme-labeled anti-immunoglobulin)

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16
Q

content of brown tube

A

substrate

17
Q

wash buffer in beaker consists of?

A

phosphate buffered saline with 0.05%; Tween 20

18
Q

tubes 1-3 is labeled as?

A

positive control

19
Q

tubes 4-6 is labeled as?

A

negative control

20
Q

tubes 7-9 is labeled as:

A

initials of patient A

21
Q

tubes 10-12 is labeled as?

A

initials of patient B

22
Q

analytical technique to SEPARATE proteins based on their MOLECULAR WEIGHT

A

SDS-PAGE

23
Q

give the 3 commonly used enzyme immunoassay in ELISA

A

ALP, HRP, B-galactosidase

MBD (5 decks)

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