FINALS: L1 - MYCOBACTERIUM

Question 1

MO TF

1. Mycobacteria - Most pathogenic species requires 2-6 weeks of incubation
2. They are Non-flagellated, non-motile, and spore formers

Answer 1

1 true
2 false

2 - non-spore formers

Question 2

MO TF

1. Mycobacteria - are acid-fast bacilli
2. Gram variable in GS

Answer 2

Both true

Question 3

MO TF

1. MTB - 729-932 doubling time
2. Affect through airborne and droplets

Answer 3

1 true
2 false

2 - cannot transmit through airborne

Question 4

MO TF

1. MTB - affects the lobar part of lungs
2. Highest concentration of oxygen is at apical portion

Answer 4

1 false
2 true

1 - apical

Question 5

MO TF

1. MTB - intracellular organisms, can survive inside macrophage
2. Everyone inhaled MTB will develop infection

Answer 5

1 true
2 false

2 - 50% will not develop infection

Question 6

MO TF

1. Antibodies and complement are ineffective against MTB
2. Complement pathways cannot be activated (MTB infection)

Answer 6

BOTH TRUE

Question 7

MO TF

1. MTB - gain entry into macrophages via cell surface molecules
2. Such as - Integrin family CR1 and CR3 complement receptors, and mannose receptors

Answer 7

BOTH TRUE

Question 8

MO TF

1. Hemoptysis - included only in the reactivation of TB
2. Coughing of blood

Answer 8

BOTH TRUE

Question 9

Lowenstein-Jensen slant - MTB appears as __________

Answer 9

Bread-crump appearance

Question 10

Lowenstein-Jensen slant - M. avium appears as

Answer 10

Condom-colony

Question 11

Tuberculin test
Select all that applies:
1. Tuberculin test - identifies tb infection, determine with/without disease
2. Identify also if it is past or recent
3. Can be done by medtech
4. Observe for swelling or induration

Answer 11

124

3 - only nurse

Question 12

1. Prepared from boiled 6-week-old broth cultures,
   from which the organisms were filtered and
   concentrated by steaming
2. The active component of the filtrate is a

Answer 12

Old tuberculin

Question 13

1. a partially purified preparation of OT prepared
   by ammonium sulfate fractionation
2. test reagent currently used for tuberculin skin
   testing

Answer 13

Purified Protein Derivative (PPD)

Question 14

Most common method of administration in Tuberculin test via intracutaneous injection

Answer 14

Mantoux test

Question 15

OT/PPD + lanolin-ointment rubbed onto skin

Answer 15

Moro percutaneous test

Question 16

T OR F
Decontamination and Digestion

Recommended technique - N-acetyl-L-cysteine (dithiothreitol) as decontaminating agent
with 4% NaOH as mucolytic agent

Answer 16

N-acetyl-L-cysteine (dithiothreitol) as mucolytic agent

with 4% NaOH(decontaminating agent)

Question 17

Mordant used in Kinyoun technique

Answer 17

Torgetol

Question 18

Report 1-2/300 fields (CDC method of reporting)

Answer 18

+/-

Question 19

Contains 0.1% casein hydrolysate, improves recovery of isoniazid resistant MTB

Answer 19

Middlebrook 7H11

Question 20

MO TF

1. Niacin test - differentiate MTB and M. bovis
2. MTB - positive (yellow color)

Answer 20

BOTH TRUE

Question 21

Medium and reagent used in catalase test - differentiate MTB and M. kansasii

Answer 21

TWEEN 80 (Medium)

30% H202 (Reagent)

Question 22

ARYLSULFATASE TEST MTB result:

Answer 22

MTB and MAC

- negative

Question 23

useful test for differentiation of M. bovis from other Mycobacteria

Answer 23

Susceptible to thiophene-2-carboxylic acid hydrazide (TCH)

Mycobacterium tubercolosis - TCH resistant

Question 24

Contains phenolase obtained from lepromatous skin nodules which differentiates it from other Mycobacteria

Answer 24

Mycobacterium leprae (Hansen’s bacillus)

Question 25

means “of the sea”, agent of

swimming pool granuloma*

Answer 25

M.marinum