finals - gene expression Flashcards
____ and mRNA that encodes several different proteins; common in bacteria
polycistronic mRNA
(T/F) polycistronic mRNAs are found in both bacteria and eukaryotes
false
it is unique in bacteria
____ preferred carbon source of E. coli
glucose
(T/F) in presence of both glucose and lactose , structural proteins of lac operon are produced
false
they are not produced
(T/F) Induction of lac operon only occurs when sole carbon source is lactose and no glucose is present
true
_____ the inhibition of lac proteins by glucose which is regulated by regulatory proteins that bind to the promoter, Plac
catabolite repression
____ a a signaling molecule generated from ATP in response to many forms of cell stimulation
cAMP
(T/F) Binding of CAP to the lac promoter depends on the concentration of adenosine-3’,5’-monophosphate (cAMP) in the bacterium
true
_____ the enzyme that catalyzes the formation of cAMP
adenylate cyclase
(T/F) the “hunger signal”
for the cell: In the absence of glucose, cAMP levels are low
false
cAMP levels are high
(T/F) the presence of cAMP-CAP complex bound to promoter, and the inducer, allolactose , RNA pol binding to the promoter is strong and transcription of the structural genes occurs
true
(T/F) in the presence of glucose, cAMP binds to CAP and the complex binds to CAP binding site on promoter
false
occurs in absence of glucose
- which of the ff is not true?
a. In absence of both glucose and lactose the operon is off because the repressor is bound
b. In presence of lactose and glucose CAP is not bound and operon is off
c. In presence of glucose and lactose, repressor is bound to operator and operon off
d. in high cAMP levels and cAMP-CAP complex at CAP site, presence of lactose to remove repressor the operon turned on
C
glucose is present but NOT lactose
(T/F) the presence of glucose inactivates adenylate cyclase
true
(T/F) The CAP site is a silencer element since it binds a transcription factor cAMP-CAP
complex and enhances transcription
false
it is and ENHANCER element
(T/F) CAP functions as a positive regulator (mediates positive regulation of transcription) since RNA pol requires its presence to function effectively
true
(T/F) Plasmids that do not contain the lac Z promoter can be used to drive the expression of
recombinant genes in bacteria
False
plasmids with lac Z
______ the inducer used to express recombinant genes in bacteria in experiments in place of allolactose
isopropylthio-galactoside
IPTG
(T/F) IPTG, like allolactose can be hydrolyzed and broken down by the cell (a
gratuitous inducer)
false
IPTG cannot be hydrolyzed so concentration does not decrease
______ the genes contained in pUC plasmid
LacZ, lacI and bla genes
(T/F) Ligation reaction in recombinant proteins is not 100% efficient so bacteria are transformed with mixture of normal and insert containing
plasmid
true
which of the ff is not incorporated into media for the blue/white screening of plasmids?
a. ampicillin
b. allolactose
c. IPTG
d. X-gal
B
____ the color indicator used in blue/white screening; a colorless reagent but forms blue color when cleaved by
β-gal
X-gal
*_____ a technique used to identify where proteins bind to DNA
DNA footprinting
(T/F) bacteria and eukaryotes have 3 types of RNA pol
false
bacteria only 1
which of the ff is a mismatch?
a. RNA pol I is located in the nucleous
b. RNA pol II is located in the neoplasm
c. RNA pol II transcribes all protein coding, snoRNA and snRNA genes
d. RNA pol III transcribes 5.8S, 18S, and 28S rRNA genes
D
this is for RNA pol 1
whichi of the ff does not apply to eukaryotic transcription?
a. has σ factors to direct enzyme to promoters
b. require a large set of transcriptional factors that assemble at the promoter with the RNA pol to initiate transcription
c. must contend with nucleosomes and higher order
chromatin packing
d. All three RNA pols contain 10 or more subunits
A
do not use sigma factors, uses transcriptional factors instead
(T/F) Structures of RNA pols I, II, and III differ but share 2 common large subunits that share sequence homology to two of the bacterial RNA pol subunits that comprise the catalytic site
true
which of the ff is not a component of Pol II promoter?
a. Enhancers or silencers
b. TATA box
c. Downstream elements
d. Initiator element
none of the above :)
(T/F) the TATA box is located at (+25)
false
it is -25
which of the ff is not found in pol II promoters?
a. TATA box
b. GC box
c. CTTC box
d. CAAT box
C
there’s no such thing!
(T/F) Most Pol II promoters have a TATA box and is necessarry for orienting/guiding pol II
true
TATA box is essential for transcription of some genes
(T/F) Those that contain TATA box typically have “ strong” Inr sequence
false
strong Inr = are “TATAless” promoters
which of the ff is not a function of general transcription factors category?
a. Function in correctly positioning Pol ll at the promoter
b. Regulate release of Pol ll from promoter to begin polymerization of the transcript
c. Enhance the transcription of specific genes
d. Assist in separating DNA strands to allow transcription initiation
C
this is done by gene-specific
transcription factors or activators