Exam 2 - lab Flashcards
_____ the inhibitor used against the substrate for alkaline phosphatase
phenyl phosphate
_____ the substrate used in alkaline phosphatase lab
p-nitrophenyl phosphate
_____ a product of hemoglobin breakdown
bilirubin
heme - biliverdin - bilirubin (free or conjugated)
____ the conjugated form of bilirubin
bilirubin glucoronide
(T/F) total bilirubin measures both free and conjugated bilirubin
true
(T/F) free bilirubin in blood is water soluble and bound to albumin
false
water INSOLUBLE, bound to albumin is true
the normal reference range for TOTAL bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2
C
the normal reference range for conjugated bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2
D
the normal reference range for free bilirubin
a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2
B
(T/F) conjugated bilirubin is normally seen circulating in peripheral blood in high concentrations
false
the reference range for the condition kernicterus
a. 1-8
b. 6-12
c. 10-14
d. ~20 mg/dl
D
the gene in pGLO plasmid that encodes beta-lactamase
a. GFP
b. bla
c. ara
d. pBAD
B
_____ is a cluster of structural genes controlled by a promoter
operon
____ a sequence of DNA to which RNA polymerase binds to initiate transcription of a gene
promoter
(T/F) the presence of arabinose alters the conformation or the protein araC which is bound to pBAD which promotes the transcription of the genes in the operon
true
____ the enzyme that converts biliverdin to bilirubin
biliverdin reductase
_____ the enzyme that converts heme to biliverdin
hemeoxygenase
_____ catalyzes the conjugation of bilirubin in the liver
UDP-glucoronyl transferase
_____ this method confirms that a transformed bacteria has taken up a plasmid with the correct DNA sequence
restriction enzyme analysis
____ method in harvesting bacteria in minipreps
centrifugation
which of the ff is a DNA mini prep volume?
a. 1-5 ml
b. 10 micrograms
c. 100 mg
d. none
B
100mg is a large prep
an OD260 reading of 1 corresponds to which of the ff
a. 50 ug/ml ss DNA
b. 10 ug/ml ss oligonucleotides
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA
C
an OD260 reading of 1 corresponds to which of the ff
a. 50 ug/ml ds DNA
b. 50 ug/ml ss DNA
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA or RNAw
A
____ the ratio that estimates the purity of nucleic acid solution and the values that correstonds to purity
OD260/OD280
- 8 = pure DNA
- 0 pure RNA
(T/F) an OD260/OD280 ratio of 1.3 indicates a pure DNA isolation
false
this is DNA contaminated with protein
(T/F) an OD260/OD280 ratio of 2.5 indicates a pure DNA isolation
false
this is RNA contaminated with protein
