Exam 2 - lab

Question 1

_____ the inhibitor used against the substrate for alkaline phosphatase

Answer 1

phenyl phosphate

Question 2

_____ the substrate used in alkaline phosphatase lab

Answer 2

p-nitrophenyl phosphate

Question 3

_____ a product of hemoglobin breakdown

Answer 3

bilirubin

heme - biliverdin - bilirubin (free or conjugated)

Question 4

____ the conjugated form of bilirubin

Answer 4

bilirubin glucoronide

Question 5

(T/F) total bilirubin measures both free and conjugated bilirubin

Answer 5

true

Question 6

(T/F) free bilirubin in blood is water soluble and bound to albumin

Answer 6

false

water INSOLUBLE, bound to albumin is true

Question 7

the normal reference range for TOTAL bilirubin

a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

Answer 7

C

Question 8

the normal reference range for conjugated bilirubin

a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

Answer 8

D

Question 9

the normal reference range for free bilirubin

a. 1-8
b. 0.2-1.0
c. 0.2-1.2
d. 0- 0.2

Answer 9

B

Question 10

(T/F) conjugated bilirubin is normally seen circulating in peripheral blood in high concentrations

Answer 10

false

Question 11

the reference range for the condition kernicterus

a. 1-8
b. 6-12
c. 10-14
d. ~20 mg/dl

Answer 11

D

Question 12

the gene in pGLO plasmid that encodes beta-lactamase

a. GFP
b. bla
c. ara
d. pBAD

Answer 12

B

Question 13

_____ is a cluster of structural genes controlled by a promoter

Answer 13

operon

Question 14

____ a sequence of DNA to which RNA polymerase binds to initiate transcription of a gene

Answer 14

promoter

Question 15

(T/F) the presence of arabinose alters the conformation or the protein araC which is bound to pBAD which promotes the transcription of the genes in the operon

Answer 15

true

Question 16

____ the enzyme that converts biliverdin to bilirubin

Answer 16

biliverdin reductase

Question 17

_____ the enzyme that converts heme to biliverdin

Answer 17

hemeoxygenase

Question 18

_____ catalyzes the conjugation of bilirubin in the liver

Answer 18

UDP-glucoronyl transferase

Question 19

_____ this method confirms that a transformed bacteria has taken up a plasmid with the correct DNA sequence

Answer 19

restriction enzyme analysis

Question 20

____ method in harvesting bacteria in minipreps

Answer 20

centrifugation

Question 21

which of the ff is a DNA mini prep volume?

a. 1-5 ml
b. 10 micrograms
c. 100 mg
d. none

Answer 21

B

100mg is a large prep

Question 22

an OD260 reading of 1 corresponds to which of the ff

a. 50 ug/ml ss DNA
b. 10 ug/ml ss oligonucleotides
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA

Answer 22

C

Question 23

an OD260 reading of 1 corresponds to which of the ff

a. 50 ug/ml ds DNA
b. 50 ug/ml ss DNA
c. 40 ug/ml ss RNA or DNA
d. 20 ug/ml ds DNA or RNAw

Answer 23

A

Question 24

____ the ratio that estimates the purity of nucleic acid solution and the values that correstonds to purity

Answer 24

OD260/OD280

1. 8 = pure DNA
2. 0 pure RNA

Question 25

(T/F) an OD260/OD280 ratio of 1.3 indicates a pure DNA isolation

Answer 25

false

this is DNA contaminated with protein

Question 26

(T/F) an OD260/OD280 ratio of 2.5 indicates a pure DNA isolation

Answer 26

false

this is RNA contaminated with protein